Aspartase from a Bacillus subtilis

Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Lyase

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435253, 435839, C12N 988, C12N 120, C12R 1125

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active

045528462

ABSTRACT:
A mutant Bacillus subtilis, arising from spontaneous adaptation from a parent without detectable aspartase activity, produces recoverable amounts of aspartase. The micro-organism attains maximum growth and enzyme production within about 8 hours and shows excellent glucose tolerance. Aspartase is at least in part produced constitutively, but L-aspartic acid stimulates further aspartase production. The aspartase so produced is readily released from the cell upon cell wall rupture and shows adequate extra-cellular stability for further concentration and purification.

REFERENCES:
patent: 3214345 (1965-10-01), Chibata et al.
patent: 3791926 (1974-02-01), Chibata et al.
patent: 4391910 (1983-07-01), Kimura et al.

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