Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Hydrolase
Reexamination Certificate
2008-03-07
2010-02-23
Nashed, Nashaat T (Department: 1656)
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Hydrolase
C435S252300, C435S252330, C435S254110, C435S320100, C536S023200, C426S020000, C426S639000
Reexamination Certificate
active
07666652
ABSTRACT:
The invention relates to new asparaginases having improved properties, preferably improved thermotolerance, such as improved activity at high temperatures and/or improved thermostability. The invention also relates to DNA sequences encoding such improved asparaginases, their production in a recombinant host cell, as well as methods of using the asparaginases, in particular for reduction of acrylamide in foods. The invention furthermore relates to methods of generating and preparing asparaginase variants having improved properties.
REFERENCES:
patent: 1 704 782 (2006-09-01), None
patent: WO2004/026042 (2004-04-01), None
patent: WO2004/030468 (2004-04-01), None
patent: WO2004/032648 (2004-04-01), None
Lubkowski et al., “Atomic resolution structure ofErwinia chrysanthemiL-asparaginase”, Acta Cryst. D, vol. 59, pp. 84-92 (2003).
Watanabe, K. et al. “Designing Thermostable Proteins: Ancestral Mutants of 3-Isopropylmalate Dehydrogenase Designed by suing a Phylogenetic Tree”, Journal of Molecular Biology, vol. 335, pp. 664-674 (2006.
Kotzia et al, Journal of Biotechnology, vol. 119, pp. 309-323 (2005).
Liu et al, Protein Engineering, vol. 16, No. 1, pp. 19-25 (2003).
Friis Esben Peter
Matsui Tomoko
Yamagishi Akihiko
McNamara Kristin J.
Nashed Nashaat T
Novozymes A/S
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