Ascorbic acid determination

Details Ascorbic acid determination Ascorbic acid determination

1976-08-23

1979-07-10

Naff, David M.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving oxidoreductase

G01N 3114

Patent

active

041606965

ABSTRACT:
Ascorbic acid in a sample such as blood serum is quantitatively assayed by converting the ascorbic acid to dehydroascorbic acid with ascorbic acid oxidase and reacting the dehydroascorbic with a coupling substance such as phenylenediamine to produce a detectable product. The ascorbic acid oxidase and coupling substance can be combined to form a reagent composition which can be incorporated into integral multilayer elements to form a test element.

REFERENCES:
patent: 3992158 (1976-11-01), Przybylowicz
Deutsch et al., J. of the A.O.A.C., vol. 48, No. 6, 1965, pp. 1248-1256.
Archibald, J. Biol. Chem. 158, (1945), pp. 347-373.

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