Ascorbate resistant composition, test device and method for dete

Details Ascorbate resistant composition, test device and method for dete Ascorbate resistant composition, test device and method for dete

1979-10-15

1981-09-08

Hruskoci, Peter A.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving glucose or galactose

23230B, 23901, 23932, 252408, 435 4, 435 28, 435805, 435810, C12Q 128, C12Q 154

Patent

active

042885418

ABSTRACT:
A composition, device and method for determining the presence of a component in a test sample which contains an interfering substance, such as ascorbic acid, are disclosed. The composition comprises an oxidase or other substance capable of producing peroxide in the presence of the component, a peroxidatively active substance, a chromogen capable of providing a detectable response (such as a color change) in the presence of peroxide and a peroxidatively active substance, and a complex of mercuric ion and one or more ligands wherein (a) the ligand binds covalently to the ion to form a water-soluble complex, and the ligand is not oxidizable by the complexed ion, and (b) the complex has a stability constant, K.sub.s, of at least 10.sup.7 and is substantially noninterfering with respect to the detectable response.

REFERENCES:
patent: 3266868 (1966-08-01), Harvill
patent: 3367842 (1968-02-01), Rupe et al.
patent: 3411887 (1968-11-01), Ku

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