Ascorbate oxidase, gene encoding the same, process for producing

Details Ascorbate oxidase, gene encoding the same, process for producing Ascorbate oxidase, gene encoding the same, process for producing

1995-05-11

1997-03-18

Jacobson, Dian C.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Oxidoreductase

426541, 426656, 435 25, 435 28, 4352523, 43525411, 4351723, 4353201, 43525421, 4352545, 4352543, 435325, 536 232, 935 14, C12N 902, C12N 1553, C12Q 126, A23J 100

Patent

active

056122080

ABSTRACT:
The present invention provides a novel ascorbate oxidase (ASOD) which catalyzes oxidation reaction of L-ascorbic acid with molecular oxygen to form L-dehydroascorbic acid and hydrogen peroxide, a process for producing the ascorbate oxidase comprising using a microorganism belonging to the genus Eupenicillium, a gene encoding ASOD, a transformant containing such a gene, a process for producing ASOD using such a transformant, and a reagent composition comprising ASOD, such as a reagent composition for examination, a food additive, and a reagent composition in the fields of food and clinical examination. The ascorbate oxidase has excellent stability particularly in a liquid state.

REFERENCES:
Ward, Jim, The Enzymatic Oxidation of Ascorbic Acid in the Slime Mold, Physarum Polycephalum, Plant Physiology, vol. 30, 1955, Botanical Lab., Univ. of Pennsylvania, Philadelphia, PA pp. 58-67.
Ohkawa et al., Primary Structure of Cucumber (Cucumis sativus) Ascorbate Oxidase Deduced from cDNA Sequence: Homology with Blue Copper Proteins and Tissue-Specific Expression, Proceedings of the National Academy of Sciences of USA, vol. 86, No. 4, pp. 1105-1430, Feb. 1989.
Abstract, Derwent Publication, Section Ch, Week 9416, JP-A-06-078-766, Mar. 22, 1994.

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