Ascorbate interference-resistant composition, device and method

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving oxidoreductase

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435 4, 435 10, 435 11, 435 14, 435 25, 436 66, 436 73, 436 74, 436800, 436904, C12Q 128, G01N 3320

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active

052643484

ABSTRACT:
A new and improved test device and method of determining the presence or concentration of a predetermined analyte, such as glucose, cholesterol or occult blood, in a test sample are disclosed. The test device includes a test pad comprising a suitable carrier matrix incorporating an indicator reagent composition capable of interacting with the predetermined analyte to produce a detectable or measurable response. In addition, the new and improved indicator reagent composition, comprising an indicator dye, such as a redox indicator, like a benzidine indicator; a redox mediator, such as an oxidase enzyme capable of interacting with the predetermined analyte and a peroxidase enzyme; a metal ion complex; and a cooxidant selected from the group consisting of bromate ion, chlorate ion, perchlorate ion, chromate ion, an organic oxidant like a peroxide, a hydroperoxide or a N-halo compound, and combinations thereof, is incorporated into the carrier matrix to provide a more accurate, ascorbate interference-resistant assay of a test sample for the predetermined analyte.

REFERENCES:
patent: 5079140 (1992-01-01), Albarella et al.
Birk, Inorg. Chem., vol. 12, pp. 2468-2472, 1973.
Birk et al., Inorg. Chem., vol. 17, pp. 1186-1191, 1978.
Kozub, Ph.D. Dissertation, Xerox University Microfilms, Ann Arbor, 1975.

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