Organic compounds -- part of the class 532-570 series – Organic compounds – Nitrogen attached directly or indirectly to the purine ring...
Reexamination Certificate
2000-06-12
2003-07-15
Bernhardt, Emily (Department: 1624)
Organic compounds -- part of the class 532-570 series
Organic compounds
Nitrogen attached directly or indirectly to the purine ring...
C544S230000, C544S360000, C544S372000, C544S373000, C544S392000
Reexamination Certificate
active
06593474
ABSTRACT:
This invention relates to a series of arylsubstituted piperazines, pharmaceutical compositions containing them and intermediates used in their manufacture. The compounds of the invention selectively inhibit binding to the &agr;-1
a
adrenergic receptor, a receptor which has been implicated in benign prostatic hyperplasia. In addition, compounds of the invention reduce intraurethral pressure in an in vivo model. As such the compounds are useful in the treatment of this disease.
BACKGROUND
Benign prostatic hyperplasia (BPH), a nonmalignant enlargement of the prostate, is the most common benign tumor in men. Approximately 50% of all men older than 65 years have some degree of BPH and a third of these men have clinical symptoms consistent with bladder outlet obstruction (Hieble and Caine, 1986). In the U.S., benign and malignant diseases of the prostate are responsible for more surgery than diseases of any other organ in men over the age of fifty.
There are two components of BPH, a static and a dynamic component. The static component is due to enlargement of the prostate gland, which may result in compression of the urethra and obstruction to the flow of urine from the bladder. The dynamic component is due to increased smooth muscle tone of the bladder neck and the prostate itself (which interferes with emptying of the bladder) and is regulated by alpha 1 adrenergic receptors (&agr;1-ARs). The medical treatments available for BPH address these components to varying degrees, and the therapeutic choices are expanding.
Surgical treatment options address the static component of BPH and include transurethral resection of the prostate (TURP), transurethral incision of the prostate (TUIP), open prostatectomy, balloon dilatation, hyperthermia, stents and laser ablation. TURP is the preferred treatment for patients with BPH and approximately 320,000 TURPs were performed in the U.S. in 1990 at an estimated cost of $2.2 billion (Weis et al., 1993). Although an effective treatment for most men with symptomatic BPH, approximately 20-25% of patients do not have a satisfactory long-term outcome (Lepor and Rigaud, 1990). Complications include retrograde ejaculation (70-75% of patients), impotence (5-10%), postoperative urinary tract infection (5-10%), and some degree of urinary incontinence (2-4%) (Mebust et al., 1989). Furthermore, the rate of reoperation is approximately 15-20% in men evaluated for 10 years or longer (Wennberg et al., 1987).
Apart from surgical approaches, there are some drug therapies which address the static component of this condition. Finasteride (Proscar®, Merck), is one such therapy which is indicated for the treatment of symptomatic BPH. This drug is a competitive inhibitor of the enzyme 5a-reductase which is responsible for the conversion of testosterone to dihydrotestosterone in the prostate gland (Gormley et al., 1992). Dihydrotestosterone appears to be the major mitogen for prostate growth, and agents which inhibit 5a-reductase reduce the size of the prostate and improve urine flow through the prostatic urethra. Although finasteride is a potent 5a-reductase inhibitor and causes a marked decrease in serum and tissue concentrations of dihydrotestosterone, it is only moderately effective in treating symptomatic BPH (Oesterling, 1995). The effects of finasteride take 6-12 months to become evident and for many men the clinical improvement is minimal (Barry, 1997).
The dynamic component of BPH has been addressed by the use of adrenergic receptor blocking agents (&agr;1-AR blockers) which act by decreasing the smooth muscle tone within the prostate gland itself. A variety of &agr;1-AR blockers (terazosin, prazosin, and doxazosin) have been investigated for the treatment of symptomatic bladder outlet obstruction due to BPH, with terazosin (Hytrin, Abbott) being the most extensively studied. Although the &agr;1-AR blockers are well-tolerated, approximately 10-15% of patients develop a clinically adverse event (Lepor, 1995). The undesirable effects of all members of this class are similar, with postural hypotension being the most commonly experienced side effect (Lepor et al., 1992). In comparison to the 5a-reductase inhibitors, the a1-AR blocking agents have a more rapid onset of action (Steers, 1995). However, their therapeutic effect, as measured by improvement in the symptom score and the peak urinary flow rate, is moderate. (Oesterling, 1995)
The use of &agr;1-AR antagonists in the treatment of BPH is related to their ability to decrease the tone of prostatic smooth muscle, leading to relief of the obstructive symptoms. Adrenergic receptors are found throughout the body play a dominant role in the control of blood pressure, nasal congestion, prostrate function and other processes (Harrison et al., 1991). However, there are a number of cloned &agr;1-AR receptor subtypes: &agr;1
a
-AR, &agr;1
b
-AR and &agr;1
d
-AR (Bruno et al., 1991; Forray et al., 1994; Hirasawa et al., 1993; Ramarao et al., 1992; Schwinn et al., 1995; Weinberg et al., 1994). A number of labs have characterized the &agr;1-ARs in human prostate by functional, radioligand binding, and molecular biological techniques (Forray et al., 1994; Hatano et al., 1994; Marshall et al., 1992; Marshall et al., 1995; Yamada et al., 1994). These studies provide evidence in support of the concept that the &agr;1
a
-AR subtype comprises the majority of &agr;1-ARs in human prostatic smooth muscle and mediates contraction in this tissue. These findings suggest that the development of a subtype-selective &agr;1
1
-AR antagonist might result in a therapeutically effective agent with greater selectivity for the treatment of BPH.
SUMMARY OF THE INVENTION
The invention relates to compounds of Formula I
wherein:
A is (CH
2
)
n
where n is 1-6;
R
1
is C
1-6
alkyl, phenyl,
substituted phenyl
where the phenyl substituents are independently selected from one or more of the group consisting of C
1-5
alkyl, C
1-5
alkoxy and halogen, phenylC
1-5
alkyl, or
substituted phenylC
1-5
alkyl
where the phenyl substituents are independently selected from one or more of the group consisting of C
1-5
alkyl, C
1-5
alkoxy and halogen;
R
2
is hydrogen, C
1-6
alkyl, C
1-5
alkenyl, C
2-5
alkenyl, phenylC
2-5
alkynyl, or substituted phenylC
1-5
alkyl
where the phenyl substituents are independently selected from one or more of the group consisting of C
1-5
alkyl, C
1-5
alkoxy and halogen;
E is
where:
m is 1-5;
R
3
is hydrogen, C
1-6
alkyl or oxygen,
where if R
3
is oxygen, the hashed line represents a bond and if R
3
is C
1-6
alkyl the hashed line is absent;
R
4
oxygen, hydrogen, C
1-5
alkyl, formyl, carboxy, C
1-5
alkylcarbonyl, C
1-5
alkoxycarbonyl, phenylC
1-5
alkoxy, substituted phenylC
1-5
alkoxy
where the phenyl substituents are independently selected from one or more of the group consisting of C
1-5
alkyl, C
1-5
alkoxy and halogen, amide, and substituted amide
where the nitrogen substituents are independently selected from one or more of the group consisting or hydrogen, C
1-5
alkyl, C
1-5
alkoxy and hydroxy,
where if R
4
is oxygen, the dashed line represents a bond and if R
4
is any other substituent, the dashed line is absent;
R
5
is hydrogen, C
1-5
alkyl or taken together with R
6
to form a cyclohexane, cyclopentane or cyclopropane ring;
R
6
is hydrogen, C
1-5
alkyl or taken together with R
5
to form a cyclohexane, cyclopentane or cyclopropane ring;
and pharmaceutically acceptable salts thereof.
These compounds are useful as adrenergic receptor modulating agents. The compounds of this invention selectively bind to the &agr;1
a
-AR receptor, modulate the activity of said receptor and are selective for prostate tissue over aortic tissue. As such, they represent a viable treatment for &agr;1
a
-AR receptor modulated disorders which include but are not limited to BPH.
In addition this invention contemplates pharmaceutical compositions containing compounds of Formula I, and methods of treating disorders mediated by &agr;1
a
-AR receptor with compounds of Formula I.
Aside from compounds of Formula I, this invention contemplates interme
Jolliffe Linda
Li Xiaobing
Maryanoff Cynthia
Mulcahy Linda
Murray William
Bernhardt Emily
Hope Ruby T.
Ortho-McNeil Pharmaceutical , Inc.
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