Artificial zona pellucida for in vivo culture of nude blastomere

Details Artificial zona pellucida for in vivo culture of nude blastomere Artificial zona pellucida for in vivo culture of nude blastomere

1987-11-25

1993-12-21

Chambers, Jasemine C.

Chemistry: molecular biology and microbiology

Apparatus

Differentiated tissue perfusion or preservation apparatus

600 33, 600 34, 604 29, C12M 300

Patent

active

052720861

ABSTRACT:
An artificial zona pellucida is provided for implantation in the peritoneal cavities of small laboratory animals. The assembly includes a hydrogel cartridge for containing one or more nude isolated blastomeres which can be cultured therein to an implant stage in the peritoneal cavity of a small animal such as a mouse. The combination is particularly advantageous for culturing of nude isolated blastomeres of a species different from the host small laboratory animal, such as blastomeres of domestic animals.

REFERENCES:
Wisniewski et al., In "Hydrogels for Medical & Related Applications", ed. Andrade, Amer. Chem. Soc. Symp. Series 31: 80-87 (1976).
Lee et al., J. Bioengineering 2: 269-278 (1978).
Ronel et al., J. Biomed. Materials Res. 17: 855-864 (1983).
Klomp et al., J. Biomed. Mat. Res. 17: 865-871 (1983).
Walladsen, Nature 277: 298-300 (1979).
Pinchuk et al., J. Biomed. Mat. Res. 15: 183-189 (1981).

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