Artificial transposons

Details Artificial transposons Artificial transposons

1996-06-10

1998-12-01

LeGuyader, John L.

Chemistry: molecular biology and microbiology

Vector, per se

435 6, 435 9141, 4351721, 4351723, 536 237, C12N 1565, C12N 1570, C12N 1511, C07H 2104

Patent

active

058437720

ABSTRACT:
We have developed efficient methods of creating artificial transposons and inserting these transposons into DNA targets in vitro, primarily for the purpose of mapping and sequencing DNA. A target DNA has been engineered to convert virtually any DNA sequence, or combination of sequences, into an artificial transposon; hence, custom transposons containing any desired feature can be easily designed and constructed. Such transposons are then efficiently inserted into DNA targets, in vitro, using the integrase activity present in yeast Ty1 virus-like particles. Primers complementary to the transposon termini can be used to sequence DNA flanking any transposon insertion.

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