Chemistry: molecular biology and microbiology – Virus or bacteriophage – except for viral vector or...
Patent
1997-07-22
1999-11-30
Eisenschenk, Frank C.
Chemistry: molecular biology and microbiology
Virus or bacteriophage, except for viral vector or...
435 71, 435 4, 435 5, 435236, 435239, 4351736, C12N 700
Patent
active
059941154
ABSTRACT:
Artificial systems for the production of infectious human papillomavirus allow development of diagnostic and therapeutic strategies based on replication and infectivity studies for diseases caused by these viruses. The artificial introduction of papillomavirus genomic DNA into an epithelial cell line. In particular, HPV type 18 genomic DNA is transfected into primary keratinocytes. Transfection is followed by clonal expansion of the transfected cells, and induction of epithelial stratification and differentiation in organotypic cultures resulting in the synthesis of virions. Virus particles synthesized were approximately 50 nanometers in diameter as observed by electron microscopy. In particular HPV18 virions produced by the system and purified by an isopycnic gradient were capable of infecting keratinocytes.
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Eisenschenk Frank C.
Monahan Thomas J.
Salimi Ali R.
The Penn State Research Foundation
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