Chemistry: molecular biology and microbiology – Animal cell – per se ; composition thereof; process of... – Primate cell – per se
Patent
1994-09-23
1997-12-09
Rollins, John W.
Chemistry: molecular biology and microbiology
Animal cell, per se ; composition thereof; process of...
Primate cell, per se
435371, 435402, 435325, 435 11, C12N 508
Patent
active
056959966
ABSTRACT:
The present invention provides an artificial organ system comprising an endothelial cell layer, an epithelial cell layer and an artificial microporous membrane disposed between and indirect contact with the endothelial cell layer and epithelial cell layer such that the membrane has an endothelial side and an epithelial side. Also provided is a method of constructing an artificial organ system, comprising the steps of placing an artificial microporous membrane into a tissue culture well and supporting the membrane a selected distance from a bottom of the well to create an upper and lower chamber in the well such that the membrane has an endothelial side facing the bottom of the well and an opposite epithelial side; placing endothelial cells into the upper chamber of the well under conditions such that the endothelial cells form a confluent layer of cells on the epithelial side of the membrane; and placing epithelial cells into the upper chamber of the well under conditions such that the endothelial cells migrate through pores in the membrane and attach to the endothelial side of the membrane to form a confluent layer of endothelial cells on the endothelial side of the membrane and the epithelial cells form a confluent layer of epithelial cells on the epithelial side of the membrane.
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Birkness Kristin A.
Quinn Frederick D.
Prats Francisco C.
Rollins John W.
The United States of America as represented by the Department of
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