Drug – bio-affecting and body treating compositions – Designated organic active ingredient containing – Peptide containing doai
Patent
1992-03-20
1995-05-16
Schain, Howard E.
Drug, bio-affecting and body treating compositions
Designated organic active ingredient containing
Peptide containing doai
530353, 530356, C07K 1520, A61K 3712, A61K 900
Patent
active
054160740
DESCRIPTION:
BRIEF SUMMARY
BACKGROUND OF THE INVENTION
1. Field of the Invention
The invention relates to compositions of protein materials more particularly having properties of resiliency and robustness. It also relates to their preparation and their biological, biochemical and pharmacological applications, more particularly for the making of artificial biological membranes.
In its most widely accepted sense, the term "artificial biological membrane" covers membranes obtained from products of biological origin and used in very different fields, such as surgery, medicine, pharmacy, but also in the chemical, textile or paper-making industries.
The properties required for these applications are mainly properties of resilience, satisfactory mechanical behaviour, chemical resistance and compatibility with different media.
2. Related Art
As a constituent of such membranes it has already been suggested to use elastin, taking advantage of its properties of resilience and its both hydrophilic and hydrophobic nature.
For example, French Patent Application No. 85 03057 of 1 Mar. 1985 in the name of INSERM mentions a product based on elastin and soluble fibrin (i.e., fibrin monomers) which is particularly suitable for the making of biomaterials and support materials, such as artificial tissues or supports for cell cultures.
SUMMARY AND OBJECTS OF THE INVENTION
The search for materials more particularly adapted for carrying out grafts on narrow vessels led the Inventor of the Application to study the behaviour of different biological products in relation to a web of polymeric material acting as a support.
The work carried out has shown that reacting elastin or elastin derivatives in certain conditions with another protein of the connective tissue it is possible to obtain a protein material which adheres strongly to the support material used, has the properties of structural proteins and thus particularly satisfactorily meets the demands made on biological membranes.
It is therefore an object of the invention to provide novel compositions of protein materials which have important properties of resilience and robustness and if necessary of adhesion and retain their properties with various additives.
Another aim of the invention is to provide a process enabling said compositions to be readily obtained.
Yet another object of the invention is to supply biological membranes which can more particularly be used as artificial connective tissues and as support materials for cell cultures.
The compositions of protein materials according to the invention are essentially as obtained by the reaction of a concentrated aqueous solution of type I and/or III collagen and elastin or water-soluble elastin peptides having a molecular weight (MW) greater than approximately 10 000, as obtained by the solubilization of elastin.
In the following description the aforementioned elastin peptides will be referred to as "solubilized elastin peptides" and denoted by the abbreviation SEP.
Such a composition has properties of resilience and mechanical behaviour which enable it to be compared with structural proteins. It also has high adhesive properties which are particularly valuable for use in the biological applications envisaged.
A preferred composition of protein material is essentially as obtained by the reaction of elastin and type I and type III collagen. The proportion of type III collagen in relation to that of type I collagen is advantageously higher than 50% by weight, more particularly at least 70% by weight (dry weight). It will be noted that type III collagen as commercially available contains such quantities of type I collagen.
The elastin is an elastin of human or animal origin native or mature, or possibly their derivatives. It is generally elastin extracted from animal tissues, more particularly bovine nape ligament or porcine, bovine or human aorta.
Another preferred composition according to the invention is essentially as obtained by reacting solubilized elastin peptides and type III collagen.
According to one feature of the invention the collagen use
REFERENCES:
patent: 4347234 (1982-08-01), Wahlig et al.
patent: 4600533 (1986-07-01), Cher
patent: 4776853 (1988-10-01), Klement et al.
patent: 5223490 (1993-06-01), Rabaud et al.
Kleinman et al., Analytical Biochemistry, 166, 1-13 (1987).
Murphy et al., Laboratory Investigation, 63, #3, 305-316 (1990).
Hansbrough et al., JAMA, 262, #15, Oct. 20, (1989).
Olivera et al., 22, 109-114, (1989).
Lefebvre Francoise
Rabaud Michel
Institut National de la Sante et de la Recherche Medicale
Schain Howard E.
Touzeau P. L.
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