Article having an organic dye and a monolayer of dried mammalian

Details Article having an organic dye and a monolayer of dried mammalian Article having an organic dye and a monolayer of dried mammalian

1991-05-21

1993-03-09

Saunders, David

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

422 57, 422 58, 422 61, 435 4, 435 721, 435 724, 435174, 435177, 435180, 435260, 435969, 436518, 436519, 436520, 436521, 436522, 436531, 436800, 436809, G01N 33543

Patent

active

051926636

ABSTRACT:
A method for drying mammalian cells, such as erythrocytes, lymphocytes, leukocytes and platelets onto a solid-phase support for use in solid-phase immunoassays through use of a drying solution and an article for use in immunoassays prepared by such method. The method comprises immobilizing a monolayer of cells onto the solid-phase support by non-covalent binding. This is accomplished by staining the solid-phase support with an organic dye having a net positive charge which permits non-covalent binding of the cells which carry a net negative charge to the solid-phase support. These cells are dried or fixed to the solid-phase support by addition of a drying solution which comprises an aqueous solution of a monosaccharide, disaccharide, trisaccharide or cyclitol and a salt. The preferred monosaccharide is D-(-)glucose and the preferred salt is sodium chloride. The preferred drying solution comprises a 1.0 M solution of dextrose and a 154 mM solution of sodium chloride. The drying solution is contacted with the immobilized cells and immediately decanted and any excess water or drying solution is removed by drying the solid-phase support in a sealed pouch with a dessicant material for 3-8 days at between 2.degree.-8.degree. C. The preferred amount of time required for complete drying is 7 days at 2.degree.-8.degree. C.

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