Stock material or miscellaneous articles – Hollow or container type article – Polymer or resin containing
Patent
1983-10-03
1987-07-21
Marantz, Sidney
Stock material or miscellaneous articles
Hollow or container type article
Polymer or resin containing
428173, 4284111, 428507, 428518, 428520, 428532, 435 7, 435293, 436507, 436529, 436531, 436538, 436539, 436807, 436809, G01N 33536, G01N 33539
Patent
active
046817824
ABSTRACT:
An immunoassay of a specimen of a serum or the like to determine the composition of immune complexes, includes producing on a plastic base a layer of a non-proteinaceous, non-ionic polymer which will adhere to the plastic base and has the capability of adsorbing immune complexes of the specimen, placing a specimen on the layer and treating the layer to produce an indication of the composition of the immune complexes. The polymer may be polyethylene glycol, dextran, polyvinyl chloride, a polymeric polyol or an adduct of polyethylene glycol or mixtures thereof. Washing with conventional solutions, addition of monoclonal and/or polyclonal antibodies coupled with an enzyme and addition of a substrate reactive therewith to determine the antigen component, are similar to the ELISA test, with color measurement as by spectrophotometer. Or, the addition of anti IgG-I.sup.125 and measurement by a scintillation counter may be used. Addition of IgG, IgA, IgE, IgG.sub.4, or IgM is useful in determining the class or subclass of the antibodies of the circulating immune complex. The polyethylene glycol may range in molecular weight from 2,000 to 20,000, although 6,000 to 8,000 is preferred. A product for use in such an assay is a plate having wells or a test tube formed of plastic, polystyrene and polyvinyl chloride being preferred, with a layer of such non-proteinaceous, non-ionic layer on the plate wells or the cavity of the test tube. This product, if it is not to be used immediately, should be provided with a layer or film protecting the polymer layer from the air. The product may also be a solid non-proteinaceous, non-ionic plastic, capable of adsorbing immune complexes from a serium sample.
REFERENCES:
patent: 4246319 (1981-01-01), Jacobone
patent: 4450231 (1984-05-01), Ozkan
Berkstresser Jerry W.
Biostar Medical Products, Inc.
Marantz Sidney
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