Arrays of probes for positional sequencing by hybridization

Details Arrays of probes for positional sequencing by hybridization Arrays of probes for positional sequencing by hybridization

2008-01-15

2008-01-15

Forman, BJ (Department: 1634)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

C435S174000, C435S283100, C435S287200, C536S023100, C536S024300

Reexamination Certificate

active

07319003

ABSTRACT:
This invention is directed to methods and reagents useful for sequencing nucleic acid targets utilizing sequencing by hybridization technology comprising probes, arrays of probes and methods whereby sequence information is obtained rapidly and efficiently in discrete packages. That information can be used for the detection, identification, purification and complete or partial sequencing of a particular target nucleic acid. When coupled with a ligation step, these methods can be performed under a single set of hybridization conditions. The invention also relates to the replication of probe arrays and methods for making and replicating arrays of probes which are useful for the large scale manufacture of diagnostic aids used to screen biological samples for specific target sequences. Arrays created using PCR technology may comprise probes with 5′- and/or 3′-overhangs.

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