Array-type multiple cell injector

Chemical apparatus and process disinfecting – deodorizing – preser – Control element responsive to a sensed operating condition

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422102, 435287, 435293, 435294, 435301, 436 63, 436165, 436180, 436809, C12M 126

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052621286

ABSTRACT:
An injection device injects small amounts of injectate into each of a plurality of cells. A first plate, which may be made from a silicon chip, includes a large number of evenly spaced cell wells, in a regular array. Each well includes a through hole in the chip. A top plate, also formed from a silicon chip, includes a plurality of injection needles, arranged in a regular array corresponding to the position of the cell wells. A suspension of cells is washed over the first plate and individual cells are retained by the individual wells. A vacuum may be applied to the cell wells through a manifold beneath the first plate, thus to retain the cells in place. Excess cells and suspension are removed by washing the plate. The second plate, including the injection needles, is positioned on the first plate so that the needles pierce the plurality of cells retained in the wells, thus simultaneously introducing the injectate into a large number of individual cells.

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patent: 4695547 (1987-09-01), Hilliard et al.
"Fabrication of Microstructures Using the Liga Process", by W. Ehrfeld, et al., Proceedings of the IEEE Micro-Robots and Teleoperators Workshop, Hyannis, Mass., 1987, IEEE Catalog No. 87TH0204-8.

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