Array of oligonucleotides on a solid substrate

Details Array of oligonucleotides on a solid substrate Array of oligonucleotides on a solid substrate

1992-09-30

1995-08-29

Kepplinger, Esther M.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 792, 435969, 435973, 436518, 436527, 436807, 436809, 536 253, 428426, 428532, C12Q 168, G01N 33543

Patent

active

054459346

ABSTRACT:
A method and apparatus for preparation of a substrate containing a plurality of sequences. Photoremovable groups are attached to a surface of a substrate. Selected regions of the substrate are exposed to light so as to activate the selected areas. A monomer, also containing a photoremovable group, is provided to the substrate to bind at the selected areas. The process is repeated using a variety of monomers such as amino acids until sequences of a desired length are obtained. Detection methods and apparatus are also disclosed.

REFERENCES:
patent: 4562157 (1985-12-01), Lowe
patent: 4689405 (1987-08-01), Frank et al.
patent: 4728591 (1988-03-01), Clark et al.
patent: 4886741 (1989-12-01), Schgwartz
patent: 4888278 (1989-12-01), Singer et al.
patent: 4923901 (1990-05-01), Koester et al.
patent: 4973493 (1990-11-01), Guire
patent: 5079600 (1992-01-01), Schnur et al.
patent: 5143854 (1992-09-01), Pirrung et al.
patent: 5202231 (1993-04-01), Drmanac et al.
BioRad Chromatography, Electrophoresis, Immunochemistry, Molecular Biology, HPLC Catalogue M 1987 p. 182.
Hames & Heggins (ed). Nucleic Acid Hybridization: A Practical Approach (1985) IRL Press Oxford England.
Science, 253, p. 1489, Sep. 27, 1991 "Will DNA Chip Speed Genome Initiative?"
Khrapko et al., FEB, 256, pp. 118-122, Oct. 1989 "An oligonucleotide hybridization approach to DNA sequencing".
Mirzabekov, Tibtech, 12, pp. 27-32, Jan. 1994 "DNA sequencing by hybridization--a megasequencing method and a diagnostic tool?"
Southern et al., Genomics, 13, pp. 1008-1017, 1992 "Analyzing and Comparing Nucleic Acid Sequences by Hybridization to Arrays of Oligonucleotides: Evaluation Using Experimental Models".
Haridasan et al., "Peptide Synthesis Using Photolytically Cleavable 2-Nitrobenzyloxycarbonyl Protecting Group," Proc. Indian Natl. Sci. Acad., Part A (1987) 53:717-728.
Sze/McGillis, VLSI Technology, Chapter 7, pp. 267-301, McGraw-Hill, 1983.
Geysen et al., "Strategies for epitope analysis using peptide synthesis," J. Immunol. Meth. (1987) 102:259-274.
Furka et al., "More Peptides by Less Labor," Abstract No. 288 from Xth International Symposium on Medicinal Chemistry, Budapest, Hungary, Aug. 15-19, 1988.
Ohtsuka et al., "Studies on transfer ribonucleic acids and related compounds. IX(1) Ribooligonucleotide synthesis . . . 2'-hydroxyl group," Nucleic Acids Research (1974) 1:1351-1357.
Kleinfeld et al., "Controlled Outgrowth of Dissociated Neurons on Patterned Substrates," J. of Neuroscience, 8(11):4098-4120, Nov. 1988.

Affiliated with

Also associated with

Rating

Array of oligonucleotides on a solid substrate does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Array of oligonucleotides on a solid substrate, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Array of oligonucleotides on a solid substrate will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1818880