Arrangement for measuring fluorescence polarization

Details Arrangement for measuring fluorescence polarization Arrangement for measuring fluorescence polarization

1983-02-28

1985-10-22

Evans, F. L.

Optics: measuring and testing

By dispersed light spectroscopy

With sample excitation

2504612, 356 73, G01N 2164

Patent

active

045484996

ABSTRACT:
Arrangement for measuring the fluorescence polarization of cells or particles which are aligned individually along a flow stream illuminated by means of an illumination field wherein the cells are passed in succession through two light beams so as to excite fluorescence; the directions of polarization of the two excitation beams are perpendicular to one another; a pair of detector channels are provided to successively record the two polarization components of the fluorescence at both points of excitation; and an analysis system is provided which calculates the corrected degree of fluorescence for the individual cells from the measured values provided by the detector channels.

REFERENCES:
patent: 4122348 (1978-10-01), Bruck
patent: 4203670 (1980-05-01), Bromberg
patent: 4352558 (1982-10-01), Eisert
Biophysical Journal, vol. 31, Jul. 1980, pp. 97-112.

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