Arrangement for confocal fluorescence microscopy

Details Arrangement for confocal fluorescence microscopy Arrangement for confocal fluorescence microscopy

1996-03-20

1998-05-12

Evans, F. L.

Optics: measuring and testing

By dispersed light spectroscopy

With sample excitation

2504581, G01N 2164

Patent

active

057514178

ABSTRACT:
An arrangement for confocal or quasifocal fluorescence microscopy is suggested in which, in an image beam path, there are arranged in succession, an objective arrangement for acquiring an image of a specimen to be examined, at least one scanner mirror located downstream of the objective arrangement, a tube lens, a confocal first strip diaphragm, a first spectrometer arrangement, a wavelength selection diaphragm for selection of the emission wavelength, a second spectrometer arrangement which is analogous to the first spectrometer arrangement, and a detector for acquiring the brightness distribution. In which, by means of the wavelength selection diaphragm, coupling of excitation light takes place by the reflecting back of an excitation beam path to the wavelength selection diaphragm from a source of monochromatic excitation light by a strip diaphragm which corresponds to the confocal strip diaphragm and a third spectrometer arrangement which is analogous to the first spectrometer arrangement. The image beam path and excitation beam path are constituted and matched to one another such that the light emitted from the specimen reaches the detector by way of the wavelength selection diaphragm. Excitation light from the wavelength selection diaphragm, however, is prevented from hitting the detector, but traverses the confocal strip diaphragm.

REFERENCES:
patent: 4863226 (1989-09-01), Houpt et al.
patent: 5192980 (1993-03-01), Dixon et al.
patent: 5283433 (1994-02-01), Tsien

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