Arbitrarily primed polymerase chain reaction method for fingerpr

Details Arbitrarily primed polymerase chain reaction method for fingerpr Arbitrarily primed polymerase chain reaction method for fingerpr

1995-06-06

1999-01-19

Jones, W. Gary

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 911, 435 912, C12Q 768, C12P 1934

Patent

active

058612459

ABSTRACT:
A rapid method for generating a set of discrete DNA amplification products characteristic of a genome as a "fingerprint" comprises the steps of: priming target nucleic acid of a genome with an single-stranded primer to form primed nucleic acid such that a substantial degree of internal-mismatching occurs between the primer and the target nucleic acid; amplifying the primed nucleic acid by performing at least one cycle of polymerase chain reaction amplification; and amplifying the product of step (2) by performing at least about 10 cycles of polymerase chain reaction amplification. The method is known as the arbitrarily primed polymerase chain reaction (AP-PCR) method and is suitable for the identification of bacterial species and strains, including Staphylococcus and Streptococcus species, mammals and plants. The method of the present invention can identify species rapidly, using only a small amount of biological material, and does not require knowledge of the nucleotide sequence or other molecular biology of the nucleic acids of the organisms to be identified. Only one primer sequence is required for amplification and/or identification. The method can also be used to generate detectable polymorphisms for use in genetic mapping of animals and humans.

REFERENCES:
patent: 4683195 (1987-07-01), Mullis et al.
patent: 4683202 (1987-07-01), Mullis
patent: 4963663 (1990-10-01), White et al.
patent: 4965188 (1990-10-01), Mullis et al.
patent: 5043272 (1991-08-01), Hartley et al.
patent: 5104792 (1992-04-01), Silver et al.
patent: 5126239 (1992-06-01), Livak et al.
patent: 5262311 (1993-11-01), Pardee et al.
patent: 5487985 (1996-01-01), McClelland et al.
Barry et al., Bio/Technology 8:233-236 (Mar., 1990).
Wahlberg et al. Molecular and Cellular Probes 4(4): 285-297.
Amann et al., 1990 "Flourescent-Oligonucleotide Probing of Whole Cells for Determinative, Phylogenetic, and Environmental Studies in Microbiology", J. Bact., 172:762-770.
Boerwinkle et al., 1989, "Rapid Typing of Tandemly Repeated Hypervariable Loci by the Polymerase Chain Reaction: Application to the Apolipoprotein B 3' Hypervariable Region", Proc. Natl. Acad. Sci. USA, 86:212-216.
Cinco et al., 1989, FEMS Microbiol. Immunol., 47:511-514.
DeLong et al., 1989, "Phylogenetic Stains: Ribosomeal RNA=Based Probes for the Identification of Single Cells", Science, 243:1360-1363.
Eisenach et al., 1988, "Repatitive DNA Sequences as Probes for Mycobacterium Tuberculosis", J. Clin. Microbiol., 26:2240-2245.
Fox, 1987, Ann. Rev. Genet., 21:67-91.
Goblet et al., 1989, "One-step Amplification of Transcripts in Total RNA Using the Polymerase Chain Reaction", Nucl. Acid. Res., 17(5):2144.
Jeffreys et al., 1985, Nature, 316:76-79.
Julier et al., 1990, Proc. Natl. Acad. Sci. USA, 87:4585-4589.
Litt et al., 1989, Am. J. Hum. Genet., 44:397-401.
McBride et al., 1989, Genomics, 5:561-573.
Nelson et al., 1989, Proc. Natl. Acad. Sci. USA, 86:6686-6690.
Ohyama et al., 1986, Nature, 322:572-574.
Rogers et al., 1984, Israel J. Med. Sci., 20:768:772.
Sommer & Tautz, 1989, "Minimal Homology Requirements for PCR Primers", Nucl. Acid. Res., 17(16):6749.
Void, 1985, Microbiol. Rev., 49:71-80.
Welsh et al., 1992, "Arbitrarily Primed PCR Fingerprinting of RNA", Nucl. Acid Res., 20:4695-4970.
Welsh and McClelland, 1990, "Fingerprinting Genomes Using PCR with Arbitrary Primers",Nucl. Acid. Res., 18(24):7213-7218.

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