Multicellular living organisms and unmodified parts thereof and – Method of introducing a polynucleotide molecule into or... – Nonplant protein is expressed from the polynucleotide
Reexamination Certificate
2006-02-28
2006-02-28
Mehta, Ashwin (Department: 1638)
Multicellular living organisms and unmodified parts thereof and
Method of introducing a polynucleotide molecule into or...
Nonplant protein is expressed from the polynucleotide
C435S419000, C435S468000, C435S471000, C435S483000, C435S484000, C536S023700, C800S298000
Reexamination Certificate
active
07005561
ABSTRACT:
Disclosed herein are novel methods and materials for selecting transgenic cells. Specifically exemplified herein are positive selection methods that involve conferring to cells the ability to metabolize certain compounds, preferably arabitol, ribitol, raffinose, sucrose, mannitol or combinations thereof. Accordingly, transformed cells can be selected by simply subjecting them to a medium containing such compounds. The subject invention alleviates the disadvantages and concerns of negative selection methods, such as the unnecessary killing of transformed cells and the dispersal of potentially harmful genes (e.g., antibiotic or herbicide resistant genes) into the environment. Furthermore, novel nucleotide sequences relating to theE. colirtl operon and arabitol dehydrogenase gene, and amino acid sequences relating to the gene products thereof are disclosed.
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Kane Patrick
LaFayette Peter
Parrott Wayne
Mehta Ashwin
Mora & Maire
University of Georgia Research Foundation Inc.
Van Dyke Timothy H.
Wolter Beusse Brownlee
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