Multicellular living organisms and unmodified parts thereof and – Plant – seedling – plant seed – or plant part – per se
Reexamination Certificate
2005-08-01
2010-10-19
Bui, Phuong T (Department: 1638)
Multicellular living organisms and unmodified parts thereof and
Plant, seedling, plant seed, or plant part, per se
C435S006120, C435S468000, C435S419000, C435S252300, C435S320100, C530S370000, C536S023600, C800S278000, C800S289000
Reexamination Certificate
active
07816582
ABSTRACT:
It is intended to provide a gene encoding a protein having an activity of improving a tolerance to an environmental stress such as salt, moisture, or heat stress, in particular, a tolerance to water stress; a protein having an activity of improving a tolerance to an environmental stress; a transgenic plant having an enhanced tolerance to an environmental stress, and so on. A halophyteSalicornia europaea, which has a strong activity of improving a tolerance to water stress and can grow even in a dry area with much salt accumulation, was used to search genes participating in the salt tolerance ofSalicornia europaeaby a functional screening method using anEscherichia coligene expression system, with the aim of isolating genes (cDNAs) participating in the salt-tolerance mechanism ofSalicornia europaea. As a result, it was found out that an arabinogalactan protein consisting of 427 amino acids and having partial homology to a Fasciclin-like arabinogalactan protein ofArabidopsis thalianahas a function of enhancing salt tolerance, water stress tolerance (sorbitol tolerance), and heat tolerance.
REFERENCES:
patent: 2004/0031072 (2004-02-01), La Rosa et al.
patent: WO 02/079245 (2002-10-01), None
Satoko Akatsuka et al., “Expression of Salicornia europaea Arabinogalactan Protein Enhances Slat Tolerance inEscherichia coli,” Plant Cell Physiol., vol. 46, Supplement, Mar. 2005, p. S161.
UniProt Consortium, Database Accession No. Q8H3S1, “Putative fasciclin-like arabinogalactan-protein,” UniProtKB/TrEMBL Database, XP 002457595, Mar. 1, 2003, pp. 1-2.
Schultz et al., “Using Genomic Resources to Guide Research Directions. The Arabinogalactan Protein Gene Family as a Test Case” Plant Physiol., Aug. 2002, vol. 129, pp. 1448-1463.
Yamada et al., “Expression of Mangrove Allene Oxide Cyclase Enhances Salt Tolerance inEscherichia coli, Yeast and Tobacco Cells”, Plant Cell Physiol., 2002, vol. 43, No. 8, pp. 903-910.
Akatsuka et al., “Establishment of Culture Cells System of Saltwort Salicornia Europaea”, Dai 45 Kai The Japanese Society of Plant Physiologists Nenkai Yoshi, Mar. 2004, p. 195, 2aI12(378) (in Japanese with translation).
Akatsuka Satoko
Ozeki Yoshihiro
Yamada Akiyo
Bui Phuong T
King & Spalding LLP
National University Corporation Tokyo University of Agriculture
Sonnenfeld Kenneth H.
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