Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives
Patent
1998-01-13
1999-12-21
Fox, David T.
Organic compounds -- part of the class 532-570 series
Organic compounds
Carbohydrates or derivatives
536 241, 800278, 800290, 435209, 4353201, 435419, 435468, C12N 504, C12N 1529, C12N 1556, C12N 1582
Patent
active
060050926
ABSTRACT:
The present invention discloses genetically engineered plants which display altered structure or morphology. The transgenic plants express a cell wall modulation transgene or gene construct that results in the altered structure or morphology. The altered structure or morphology can be associated with, for example, altered biomass, growth, yield, greater or less resistance to biodegradation, more or less digestible to ruminants, altered cellulose content, larger leaves
ormal hypocotyls or smaller leaves/longer hypocotyls, etc. compared to a non-transgenic plant of the same species. The cell wall modulation transgene can be any cellulose binding domain, a cellulose binding protein, or a cell wall modifying protein or enzyme such as endoxyloglucan transferase, xyloglucan endo-transglycosylase, an expansin, cellulose synthase, or a novel isolated endo-1,4-.beta.-glucanase of Arabidopsis thaliana. The invention also discloses transgenic plants containing a gene construct comprising a promoter operably linked to the cell wall modulation protein or polypeptide gene and may further comprise a sequence encoding a secretion signal peptide. In particular, the invention discloses transgenic plants containing a gene construct comprising the cel1 promoter, operably linked to the cel1 signal peptide and any cellulose binding domain. Methods for modulating plant growth by transgenic expression of a cell wall modulating protein or polypeptide are also disclosed. The present invention also discloses a novel, isolated Arabidopsis thaliana endo-1,4-.beta.-glucanase gene (cel1), its promoter (cel1 promoter) and polypeptide (Cel1) and recombinant nucleic acid vectors containing the cel1 gene with or without a secretion signal peptide sequence and/or the cel1 promoter.
REFERENCES:
patent: 5137819 (1992-08-01), Kilburn et al.
patent: 5202247 (1993-04-01), Kilburn et al.
patent: 5258502 (1993-11-01), Kuranda
patent: 5496934 (1996-03-01), Shoseyov et al.
patent: 5530187 (1996-06-01), Lamb et al.
patent: 5643791 (1997-07-01), Warren et al.
patent: 5705375 (1998-01-01), Van Ooyen et al.
Clackson et al., 1994, "In vitro selection from protein and peptide libras", Trends Biotech. 12:173-184.
Goldstein et al., 1993, "Characterization of the cellulose-binding domain of the clostridium cellulovorans cellulose-binding protein", J. Bacteriol. 175(18):5762-5768.
Odell et al., 1985, "Identification of DNA sequences required for activity of the cauliflower mosaic virus 35S promoter", Nature 313:810-812.
Scherban et al., 1995, "Molecular cloning and sequence analysis of expansins--a Highly conserved, multigene family of proteins that mediate cell wall extension in plants", PNAS USA 92:9245-9249.
Yu et al., 1996, "Affinity maturation of phage-displayed peptide ligands", Meth. Enzymol. 267:3-27.
Kim et al, Plant Mol. Biol., vol. 24, pp. 106, 107, 110, 1994.
Shani et al, Plant Mol. Biol., vol. 34, pp. 837-840, 1997.
Ohmiya et al, J. Bacteriol, vol. 171, pp. 6771-6772, 1989.
Nakamura et al, Plant Cell Physiol., vol. 36, p. 1231, 1995.
Cabib, Enric, "Chitinase from Serratia marcescens," Methods in Enzymology 161:460-462 (1988).
Greenwood et al., "Fusion to an endoglucanase allows alkaline phosphatase to bind to cellulose," EBS Letters 244(1):127-131 (1989).
Shoseyov et al., "Essential 170-kDa subunit for degradation of crystalline cellulose by Clostridium cellulovorans cellulase," Proc. Natl. Acad. Sci. USA 87:2192-2195 (1990).
Shoseyov et al., "Immobilized Endo-.beta.-glucosidase Enriches Flavor of Wine and Passion Fruit Juice," JAFCU 38(6):1387-1390 (1990).
Klyosov, Anatole A., "Trends in Biochemistry and Enzymology of Cellulose Degradation," Biochemistry 29(47):10577-10585 (1990).
Shoseyov et al., "Primary sequence analysis of Clostridium cellulovorans cellulose binding protein A," Proc. Natl. Acad. Sci. USA 89:3483-3487 (1992).
Din et al., "Non-Hydrolytic Disruption of Cellulose Fibres by the Binding Domain of a Bacterial Cellulase," Bio/Technology 9:1096-1099 (1991).
Gerngross, et al., 1993, "Sequencing of a Clostridium thermocellum gene (cipA) encoding the cellulosomal S.sub.L -protein reveals an unusual degree of internal homology," Molecular Biology, vol. 8, No. 2, pp. 325-334.
Hazlewood, et al., 1993, Gene sequence and properties of Cell, a family E endoglucanase from Clostridium thermocellum, Journal of General Microbiology, vol. 139: pp. 307-316.
Hansen, et al., 1992, "celA from Bacillus lautus PL236 Encodes a Novel Cellulose-Binding Endo-.beta.-1,4-Glucanase," Journal of Bacteriology, vol. 174, No. 11, pp. 3522-3531.
Saul, et al., 1989, "Nucleotide sequence of a gene from Caldocellum saccharolyticum encoding for exocellulase and endocellulase activity," Nucleic Acids Research, vol. 17, No. 1, p. 439.
MacKay, et al., 1986, "Structure of a Bacillus subtilis endo-.beta.-1,4-glucanase gene," Nucleic Acids Research, vol. 14, No. 22, pp. 9159-9170.
Jauris, et al., 1990, "Sequence analysis of the clostridium stercorarium celZ gene encoding a thermoactive cellulase (Avicelase I): Identification of catalytic and cellulose-binding domains," Mol. Gen. Genet., vol. 223: pp. 258-267.
Poole, et al., 1992, Identification of the cellulose-binding domain of the cellulosome subunit S1 from Clostridium thermocellum YS, FEMS Microbiology Letters, vol. 99: pp. 181-186.
Foong et al., 1991, "Nucleotide sequence and characteristics of endoglucanase gene engB from Clostridium cellulovorans," Journal of General Microbiology vol. 137:1729-1736.
Shoseyov et al., 1991, "Nucleotide Sequence of Clostridium cellulovorans gene homologous to cyclic-AMP dependent kinase," Nucleic Acids Res. vol. 19:1710.
Shoseyov et al., 1990, "Cloning of 170 dKa Clostridium cellulovorans Cellulase Subunit: An Essential Protein for the Degradation of Crystalline Cellulose," Abstracts of the Annual Meeting of the American Society for Microbiology O-24, p. 267.
Shoseyov et al., 1990, "Cloning of Clostridium Cellulovorans Endo-1, 4-.beta.-Glucanase Genes," Biochemical Biophysical Research Communications vol. 169(2):667-672.
Bowie et al., 1990, "Deciphering the Message in Protein Sequences Tolerance to Amino Acid Substitutions," Science vol. 247:1306-1310.
Sambrook et al., "Detection and Analysis of Proteins Expressed from Cloned Genes," Molecular Cloning, A Laboratory Manual, 2nd ed., pp. 18.2-18.75.
Greenwood et al., 1992, "Cellulose-binding domains: potential for purification of complex proteins," Protein Engineering vol. 5:361-365.
Ong et al., 1993, "The Cellulose-Binding Domain (CBD.sub.Cox) of an Exoglucanase from Cellulomonas fimi: Production in Escherichia coli and Characterization of the Polypeptide," Biotechnology and Bioengineering vol. 42:401-409.
Durrant et al., 1991, "The non-catalytic C-terminal region of endoglucanase E. from Clostridium thermocellum contains a cellulose-binding domain," Biochem. J. vol. 273:289-293.
Ong et al., 1989, "The cellulose-binding domains of cellulases: tools for biotechnology," TibTech vol. 7:239-243.
Poole et al., 1991, "Characterization of hybrid proteins consisting of the catalytic domains of Clostridium and Ruminococcus endoglucanases, fused to Pseudomonas non-catalytic cellulose-binding domains," Biochem. J. vol. 279:787-792.
Cosgrove, 1993, "How Do Plant Cell Walls Extend?", Plant Physiol. vol. 102:1-6.
Roberts, 1994, "The plant extracellular matrix: in a new expansive mood", Curr. Opin. Cell. Biol.vol. 6:688-694.
McQueen-Mason et al., 1995, "Expansion Mode of Action on Cell Walls: Analysis of Wall Hydrolysis, Stress Relaxation, and Binding", Plant Physiol. vol. 107:87-100.
Shoseyov et al., 1992, "The Role of Endo-1,4-Beta-Glucanase in Plant Cell Elongation. In-Vitro Studies of Peach Pollen", Acta. Hort. vol. 329:225-227.
Verma et al., 1975, "Regulation and in Vitro Translation of Messenger Ribonucleic Acid for Cellulase from Auxin-treated Pea Epicotyls", J. Biol. Chem. vol. 250:1019-1026.
Cass et al., 1990, "Isolation and characterization of a cellulase gene family member expressed during avocado fruit ripening", Mol. Gen. Genet. vol. 223:76-86.
Fischer and Bennett, 1991, "Role of Cell Wall Hydrolases in Fruit
Shani Ziv
Shoseyov Oded
Fox David T.
Mehta Ashwin
Yissum Research Development Company of the Hebrew University of
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