Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Separation or purification
Patent
1997-05-22
1999-05-25
Tsang, Cecilia J.
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
Separation or purification
530380, 530385, A61K 3514, C07K 1400, C07K 1600, C07K 1700
Patent
active
059070358
ABSTRACT:
The present invention relates to methods of purifying proteins having surface active, electron-rich amino acids using an aqueous two phase system. The methods include the use of salts and inert hydrophobic molecules, such as polymers, to produce the aqueous two phase system and the use a polymer-chelator-metal complex to purify the target proteins.
REFERENCES:
patent: 5407579 (1995-04-01), Lee et al.
Krishnamurthy et al./Conditions Promoting Metal-Catalyzed Oxidations During Immobilized Cu-Iminodiacetic Acid Metal Affinity Chromatography/Biotechnol. Prog. (1995) 11:643-650.
Chung & Arnold/Metal-Affinity Partitioning of Phosphoproteins in Peg/Dextran Two-Phase Systems/Biotechnology Ltrs. (1991) 13(8):615-620.
Wuenschell et al/Aqueous Two-Phase MEtal Affinity Extraction of Heme Proteins/Bioprocess Engineering (1990) 5: 199-202.
Porath et al./Metal Chelate Affinity Chromatography, A New Approach to Protein Fractionation/Nature (1975) 258:598-599.
Johanson/Partitioning in Aqueous Two-Phase Systems, Theory, Methods, Uses, and and applications to Biotechnology/Partitioning of Proteins/(1985) Chapter 6/161-226/ED. Walter, Brooks, Fisher/Academic Press, Inc.
Birkenmeier et al/Immobilized Metal Ion Affinity Partitioning, A Method Combining Metal-Protein Interaction and Partitioning of Proteins in Aqueous Two-Phase Systems/J of Chrom. (1991) 539:267-277.
Todd et al./Cu(II)-Binding Properties of a Cytochrome c With a Synthetic Metal-Binding Site: HIS-X.sub.3 -HIS in an .alpha.-Helix/Proteins: Structure, Function, and Genetics (1991) 10:156-161.
Plunkett et al./Metal Affinity Extraction of Human Hemoglobin in an Aqueous Polyethylene Glycol-Sodium Sulfate Two-Phase System/Biotech. Tech. (1990) 4(1):45-48.
Hart & Bailey/Purification and Aqueous Two-Phase Partitioning Properties of Recombinant Vitreoscilla Hemoglobin/Enzyme Micron. Technol. (1991) 13: 788-795.
Baxter Biotech Technology Sarl
Marshall S. G.
Tsang Cecilia J.
LandOfFree
Aqueous two-phase metal affinity partitioning protein purificati does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Aqueous two-phase metal affinity partitioning protein purificati, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Aqueous two-phase metal affinity partitioning protein purificati will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-401714