Aqueous solution as an eluent used in liquid chromatography

Chemistry: analytical and immunological testing – Including chromatography

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73 611C, A01N 3002

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046668616

ABSTRACT:
Disclosed is an aqueous solution as an eluent used in liquid chromatography for analysis of a specimen obtained from a human living body, comprising a mixture of acetonitrile and an aqueous solution of a member selected from the group consisting of acetic acid, a polybasic organic acid, a salt of organic acid and ammonium carbonate.

REFERENCES:
B. G. Belenkii et al, Modern Liquid Chromatography of Macromolecules, Journal of Chromatography Library, vol. 25, 1983, pp. 1, 2, 65-67.
Jeffrey R. Koup et al, High-Performance Liquid Chromatographic Assay of Chloramphenicol in Serum, Antimicrobial Agents and Chemotherapy, Sep. 1978, pp. 439-443.
Ronald E. Majors, Techniques for Liquid Chromatographic Columns Packed with Small Porous Particles, Analytical Chemistry, vol. 45, No. 4, Apr. 1973, pp. 755-762.
Kiyoshi Tsuji, Fluorimetric Determination of Erythromycin and Erythromycin Ethylsuccinate in Serum by a High-Performance Liquid Chromatographic Post-Column, On-Stream Derivatization and Extraction Method, Journal of Chromatography, 158 (1978) pp. 337-348.
Robert B. Hagel et al, Stability-Indicating Assay for Mecillinam Using High-Pressure Liquid Chromatography, Journal of Chromatology, 170 (1979).
D. Dell et al, The Liquid Chromatographic Analysis and Pharmacokinetics of the Semi-synthetic Cephalosporin 3-Methyl-7[4-(1,4,56-tetrahydro-2-pyrimidyl)-phenylacetamidol]-.DELTA..sup .3 Acid(1), from the Hoechst Pharmaceutical Research Laboratories, 1978, pp. 940-944.
Shigeaki Baba et al, A Measurement of Individual Bile Acids in Serum by High-Performance Liquid Chromatography for Clinical Diagnostic Information of Hepatobiliary Diseases, Kobe J. Med. Sci. 26, 89-99, Jun. 1980.

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