Chemistry: natural resins or derivatives; peptides or proteins; – Peptides of 3 to 100 amino acid residues
Reexamination Certificate
2000-01-31
2002-11-12
Low, Christopher S. F. (Department: 1653)
Chemistry: natural resins or derivatives; peptides or proteins;
Peptides of 3 to 100 amino acid residues
C530S395000, C530S400000, C530S832000, C514S002600, C560S001000, C560S125000, C560S127000
Reexamination Certificate
active
06479627
ABSTRACT:
TECHNICAL FIELD
The present invention relates to an aqueous preparation of lactoferrin where stability of lactoferrin in an aqueous solution is improved and also to a method of stabilizing lactoferrin in an aqueous solution.
BACKGROUND ART
Lactoferrin is a protein existing in milk or tears of a human being, bovine, etc. and is known to have pharmacological effects such as an antibacterial effect and a lymphocyte proliferation effect (Japanese Laid-Open Patent Publication Hei-02/48534). It is also reported that lactoferrin is useful as a therapeutic agent of corneal disorders due to its excellent promotive effects of corneal keratocytes proliferation (WO 2/08477); is useful as a preventive agent of postoperative astigmatism due to its enhancing effects of wound closure strength (Japanese Laid-Open Patent Publication Hei-08/301785); and is useful as a promotive agent of mucin production due to its excellent accelerating effects of producing proteins containing mucin (Japanese Laid-Open Patent Publication Hei-09/12473).
In addition, physiologically active protein substances such as lactoferrin can be produced in large quantities due to progress in manufacturing technique and have been widely studied for their utilization as pharmaceuticals.
However, physiologically active protein substances are generally unstable as compared with ordinary low-molecular synthetic compounds and are poorly soluble in water and very difficult to be applied to aqueous preparations.
In particular, with regard to stability, they have problems such as decomposition, deamidation, dimerization and oxidation caused by chemical factors and phase transition, aggregation in a manner of noncovalent bond, adsorption caused by physical factors whereby it is difficult to maintain their stability in aqueous preparations. Lactoferrin also has a problem that, when it is preserved for a long period of time in an aqueous solution, the content of lactoferrin remarkably lowers due to decomposition, aggregation, etc. Under such circumstances, it is quite significant to stabilize lactoferrin, which is useful as a pharmaceutical, in an aqueous solution and to develop an aqueous preparation which can be preserved for a long period of time.
DISCLOSURE OF THE INVENTION
In order to develop aqueous preparations of lactoferrin which can be preserved for a long period of time, the present inventors have studied on formulating various additives and have found that formulation of a polyvalent inorganic or organic acid or a salt thereof as an additive improves stability of lactoferrin in an aqueous solution, whereupon the present invention has been achieved.
The present invention relates to an aqueous preparation of lactoferrin having improved stability which is characterized by containing a polyvalent inorganic or organic acid or a salt thereof. The present invention also relates to a method of stabilizing lactoferrin comprising adding 0.005% (w/v) or more of a polyvalent inorganic or organic acid or a salt thereof to an aqueous solution of lactoferrin.
To be more specific, the aqueous preparation according to the present invention is characterized by containing 0.005% (w/v) or more of a polyvalent inorganic or organic acid or a salt thereof.
The polyvalent inorganic acid in the present invention stands for an inorganic acid which is capable of producing divalent or higher polyvalent anions, that is, a polybasic acid. Examples of the inorganic acid are phosphoric acid and sulfuric acid while examples of the salt are alkali salts such as sodium salt and potassium salt; alkali earth metal salts such as calcium salt; and ammonium salt. Preferred examples are sodium phosphate and sodium sulfate, and particularly preferred examples are sodium phosphate, sodium monohydrogen phosphate and sodium dihydrogen phosphate. The polyvalent inorganic acid can be used either solely or in combination thereof.
The polyvalent organic acid in the present invention stands for an organic acid which is capable of producing divalent or higher polyvalent anions. Examples of the organic acid are citric acid, maleic acid, fumaric acid, tartaric acid, succinic acid, ethylenediamine tetraacetic acid and chondroitin sulfate while examples of salts thereof are alkali salts such as sodium salt and potassium salt; alkali earth metal salts such as calcium salt; and ammonium salt. Preferred examples are sodium citrate and sodium maleate, and particularly preferred examples are sodium citrate, sodium monohydrogen citrate, sodium dihydrogen citrate, sodium maleate and sodium monohydrogen maleate.
The polyvalent organic acid can also be used either solely or in combination thereof and can be used in combination with the above-mentioned polyvalent inorganic acid.
Effects of the present invention will be described in detail in the stability test which will be mentioned later. Stability of lactoferrin in an aqueous solution is significantly improved by formulating 0.005% (w/v) or more of a polyvalent inorganic or organic acid or a salt thereof. The stability of lactoferrin increases remarkably, with increasing the concentration of the acid or its salt in the solution. Accordingly, the concentration does not need defining the upper limit and the acid or a salt thereof can be formulated up to an extent which is usually allowed for ordinary aqueous preparations. The above-mentioned inorganic or organic acid or salt thereof affects the osmotic pressure of the aqueous preparation. Aqueous preparations having too high osmotic pressure are not preferable as pharmaceuticals. In the case of ophthalmic solutions, for example, the higher the osmotic pressure, the more the irritation upon instilling them into eye. Accordingly, the acceptable upper limit of the osmotic pressure ratio is usually around two. Since the concentration of the acid or its salt varies depending upon the sort of inorganic or organic acid or a salt thereof to be formulated, it is difficult to generally define the upper limit of the concentration, but in the case of sodium dihydrogen phosphate dihydrate, for example, it is around 9.0% (w/v).
Considering a stabilizing effect and an appropriate range for osmotic pressure ratio totally, the concentration of the inorganic or organic acid or a salt thereof, in the case of Ophthalmic solutions, for example, is preferably 0.01-3.0% (w/v), more preferably 0.1-1.0% (w/v).
The concentration of lactoferrin can be appropriately selected depending upon its use and, in the case of ophthalmic solutions, for example, it is 0.01-3.0% (w/v), preferably 0.1-1.0% (w/v).
The aqueous preparation of the present invention can be widely used in life science fields including pharmaceuticals, foods and cosmetics and, particularly when it is used as pharmaceuticals, examples of the dosage form are injections, ophthalmic solutions and nasal drops which can be prepared by widely-used techniques. For example, ophthalmic solutions can be prepared using various ingredients which are commonly used for opthalmic solutions including isotonicities such as sodium chloride and concentrated glycerol; buffers such as sodium acetate buffer; nonionic surfactants such as polyoxyethylene sorbitan monooleate, polyoxyl stearate 40 and polyoxyethylene hydrogenated castor oil; and preservatives such as benzalkonium chloride and paraben. The pH can be within a range which is allowed for ophthalmic preparations and is preferably within a range of 4-8.
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Asada Hiroyuki
Kuwano Mitsuaki
Ota Atsutoshi
Frishauf Holtz Goodman & Chick P.C.
Gupta Anish
Low Christopher S. F.
Santen Pharmaceutical Co. Ltd.
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