Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Patent
1995-02-07
1998-03-03
Walsh, Stephen
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
530402, 530422, 530399, 435 701, 435803, 435804, C07K 120, C07K 1465, C07K 1461, C12N 100
Patent
active
057233101
ABSTRACT:
A method is described for isolating an exogenous polypeptide in a non-native conformation from cells, such as an aqueous fermentation broth, in which it is prepared comprising contacting the polypeptide with a chaotropic agent and preferably a reducing agent and with phase-forming species to form multiple aqueous phases, with one of the phases being enriched in the polypeptide and depleted in the biomass solids and nucleic acids originating from the cells. Preferably, the method results in two aqueous phases, with the upper phase being enriched in the polypeptide.
REFERENCES:
patent: 4565785 (1986-01-01), Gilbert
patent: 4579661 (1986-04-01), Gustafsson et al.
patent: 4673641 (1987-06-01), George et al.
patent: 4684723 (1987-08-01), Dove et al.
patent: 4690892 (1987-09-01), Ananthapadmananhan et al.
patent: 4710473 (1987-12-01), Morris
patent: 4738921 (1988-04-01), Bellagaje et al.
patent: 4795706 (1989-01-01), Hsiung et al.
patent: 4843155 (1989-06-01), Chomczynski
patent: 4879234 (1989-11-01), Cordes et al.
patent: 4961969 (1990-10-01), Hershenson et al.
patent: 5093254 (1992-03-01), Giuliano et al.
patent: 5158875 (1992-10-01), Miller et al.
patent: 5210028 (1993-05-01), Schmitz et al.
patent: 5288931 (1994-02-01), Chang et al.
patent: 5407810 (1995-04-01), Building et al.
patent: 5410026 (1995-04-01), Chang et al.
patent: 5459052 (1995-10-01), Skriver et al.
Franks et al., "The Role of Solvent Interactions in Protein Conformation" CRC Crit. Rev. Biochem. 3:165-219 (1975).
Kula, "Liquid-Liquid Extraction of Biopolymers" Comprehensive Biotechnology, A. Humphrey and C.L. Clooney, New York:Pergamon Press vol. 2:451-471 (1985).
Tanford, "Protein Denaturation--Part C--Theoretical Models for the Mechanism of Denaturation" Adv. Prot. Chem. 24:69-72 (1970).
Albertsson, "Aqueous polymer-phase systems" partition of Cell Particles and Cell Macromolecules pp. 8-39 (1986).
Albertsson, "Biotechnical applications" Partition of Cell Particles and Macromolecules pp. 212-226 (1986).
Albertsson, "Factors determining partition" Partition of Cell Particles and Cell Macromolecules pp. 56-111 (1986).
Albertsson, "History of aqueous polymer two-phase partition" Partitioning in Aqueous Two-Phase Systems, Walter et al., ed. pp. 1-10 (1985).
Baskir et al., "Thermodynamics of the separation of biomaterials in two-phase aqueous polymer systems: effect of the phase-forming polymers" Macromolecules 20:1300-1311 (1987).
Becker et al., "Downstream processing of proteins" Biotech Advs. 1:247-261 (1983).
Birkenmeier et al., "Dye-promoted precipitation of serum proteins. Mechanism and application" Journal of Biotechnology 21:93-108 (1991).
Birkenmeier et al., "Partition of purified human thyroxine-binding globulin in aqueous two-phase systems in response to reactive dyes" Journal of Chromatography pp. 193-201 (1986).
Blomquist et al., "A study of DNA from chloroplasts separated by counter-current distribution" Acta Chemica Scandinavia pp. 838-842 (1975).
Blomquist et al., "A study of extraction column for aqueous polymer two-phase systems" Journal of Chromatography pp. 125-133 (1972).
Brems D. et al., "Equilibrium denaturation of pituitary-- and recombinant-derived bovine growth hormone" Biochemistry 24(26):7662-7668 (1985).
Cole, "Alt-PEG Two-Phase Aqueous Systems to Purify Proteins and Nucleic Acid Mixtures" Frontiers BioProcess II pp. 340-351 (1992).
Cole, "Purification of Plasmid and High Molecular Mass DNA Using PEG-Salt Two-Phase Extraction" BioTechniques 11(1):18-24 (1991).
Erlanson-Albertisson, "Measurement of the binding of colipase to a triacylglycerol substrate" Biochemica et Biophysica Acta 617:371-382 (1980).
Foster et al., "Interactions of human sperm acrosomal protein SP-10 with the acrosomal membranes" Biology of Reproduction 46:981-990 (1992).
Glossmann et al., "The preparation of brush border membranes from rat kidney using an aqueous two-phase polymer system" Naunyn-Schmiedenberg's Arch. Pharmacol. 282:439-444 (1974).
Grunfield et al., "Effector-assisted refolding of recombinant tissue-plasminogen activator produced in Escherichia coli" Applied Biochemistry and Biotechnology 33(2):117-138 (1992).
Hattori et al., "Studies of the high molecular weight form of polypeptide chain elongation factor-1 from pig liver" J. Biochem 88:725-736 (1980).
Haynes et al., "Electrostatic potentials and protein partitioning in aqueous two-phase systems" AIChE Journal pp. 1401-1409 (Sep. 1991).
Hejnaes et al., "Development of an optimized refolding process for recombinant Ala-Glu-IGF-1" Protein Engineering 5(8):797-806 (1992).
Hustedt et al., "Applications of phase partitioning in biotechnology" Patitioning in Aqueous Two-Phase Systems, Walter et al., ed. pp. 529-587 (1985).
Johansson, "Partitioning of proteins" Partitioning in Aqueous Two-Phase Systems, Walter et al., ed. pp. 161-226 (1985).
Johansson et al., "Affinity partitioning of biopolymers and membranes in Ficoll-Dextran aqueous two-phase systems", Journal of Chromatography 331:11-21 (1985).
Johansson et al., "Effects of organic solvents on the partitioning of enzymes in aqueous two-phase systems" Journal of Chromatography 388(2):295-305 (1987).
Kessel et al., "Effect of dithiocarbanilates on some biological and biophysical properties of leukemia L1210 cell membranes" Molecular Pharmacology pp. 1121-1129 (1978).
Kowalczyk et al., "Enzymic synthesis of 1-o (indol-3-ylacetyl)-beta-D-glucose. Purification of the enzyme from Zea mays, and preparation of antibodies to the enzyme" Biochemical Journal 279(Pt. 2):509-514 (1991).
Kroner et al., "Evaluation of crude dextran as phase-forming polymer for the extraction of enzymes in aqueous two-phase systems in large scale" Biotechnology & Bioenineering pp. 1015-1045 (1982).
Ku et al., "Affinity-specific protein separations using ligand-coupled particles in aqueous two-phase systems: I. process concept and enzyme binding studies for pyruvate kinase and alcohol dehydrogenase from saccharomyces cerevisiae" Biotechnology & Bioengineering 33:1081-1088 (1989).
Kuboi et al., "Kagaku Kogaku Rombun Shu, Kagaku Kogaku Ronbunshu" KKRBAW (abstract only, ISSN 0386-216X) pp. 772-779 (1990).
Kuboi et al, "Kagaku Kogaku Rombun Shu, Kagaku Kogaku Ronbunshu" KKRBAW (abstract only, ISSN 0386-216X) pp. 1053-1059 (1990).
Kula et al., "Extraction processes" 8th International Biotechnology Symposium pp. 612-622 (1988).
Kula et al., "Purification of enzymes by liquid-liquid extraction" Advances in Biochemical Engineering pp. 73-118 (1982).
Lee et al., "Partitioning of recombinant interleukin-2 is a poly(ethylene-glycol)-dextran aqueous two-phase system" J. Microbiol. Biotech. (Chem. Abst. 119:7235, Abstr. #7250r (1993)) 2(2) :135-140 (1992).
Lemoine et al., "Active uptake of sucrose by plant plasma membrane vesicles: determination of some important physical and energetical parameters" Physiologica Plantarum 82:377-384 (1991).
Lillehoj et al., "Protein purification" Advances in Biochemical Engineering-Biotechnology 40:19-71 (1989).
Lundberg S. et al., "Characterization of calcium binding to spectrins" Biochemistry 31(24) :5665-5671 (Jun. 1992).
Mak, S., et al., "Purification of adenovirus messenger ribonucleic acid by an aqueous polymer two-phase system" Biochemistry 15(26) :5754-5761 (1976).
Marciani et al., "Polypeptide composition of cell membranes from chick embryo fibroblasts transformed by rous sarcoma virus" Biochimica et Biophysica Acta 401 (3) :386-398 (Sep. 1975).
Mattiasson et al., "Use of aqueous two-phase sytems for recovery and purification in biotechnology" Separ Recovery Purif: Math Model pp. 78-92 (1986).
Mendieta et al., "Affinity-mediated modification of electrical charge on cell surface: a new approach to the affinity partitioning of biological particles" Analytical Biochemistry 200(2) :280-285 (Feb. 1992).
Moudgil et al., "In vitro modulation of rat liver glucocorticoid receptor by urea" Journal of Biological Chemistry 262(11):5180-5187 (Apr. 1987).
Nifant'eva et al., "Extraction of thiocyanate and halide metal complexes in two-phase aqueous systems polyethylene glycol-salt-water" Journal of Analytical Chemistry of t
Builder Stuart
Hart Roger
Lester Philip
Ogez John
Reifsnyder David
Genentech Inc.
Hasak Janet E.
Romeo David S.
Walsh Stephen
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