Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Patent
1993-08-20
1995-04-18
Walsh, Stephen G.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
435804, 530399, 530412, 530422, 530808, C12N 121, C07K 312, C07K 328, C08H 100
Patent
active
054078105
ABSTRACT:
A method is described for isolating an exogenous polypeptide in a non-native conformation from cells, such as an aqueous fermentation broth, in which it is prepared comprising contacting the polypeptide with a chaotropic agent and preferably a reducing agent and with phase-forming species to form multiple aqueous phases, with one of the phases being enriched in the polypeptide and depleted in the biomass solids and nucleic acids originating from the cells. Preferably, the method results in two aqueous phases, with the upper phase being enriched in the polypeptide.
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Builder Stuart
Hart Roger
Lester Philip
Ogez John
Reifsnyder David
Genentech Inc.
Hasak Janet E.
Walsh Stephen G.
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