Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving cholesterol
Reexamination Certificate
2007-04-10
2007-04-10
Gitomer, Ralph (Department: 1657)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving cholesterol
Reexamination Certificate
active
10430231
ABSTRACT:
The present invention provides a simple and sensitive aqueous method for determining cholesterol in remnant-like particles in a biological sample without the separation of components of the sample. The method comprises contacting the sample with (i) cholesterol esterase, (ii) cholesterol oxidase or cholesterol dehydrogenase, and (iii) phospholipase with a surfactant that inhibits cholesterol oxidase or cholesterol dehydrogenase in lipoproteins other than remnant-like particles in the presence of oxygen or an oxidized coenzyme. Formed hydrogen peroxide or reduced coenzyme is measured and correlated to a quantitative determination of cholesterol in remnant-like particles.
REFERENCES:
patent: 5041636 (1991-08-01), Sakata et al.
patent: 5164512 (1992-11-01), Hashizume et al.
patent: 5238818 (1993-08-01), Hashizume et al.
patent: 5635468 (1997-06-01), Ara et al.
patent: 6114134 (2000-09-01), Kishi et al.
patent: 6794157 (2004-09-01), Sugiuchi
patent: 6986998 (2006-01-01), Kishi et al.
patent: 3319066 (1984-11-01), None
patent: 0 698 791 (1996-02-01), None
patent: 0 699 767 (1996-03-01), None
patent: 0 763 741 (1997-03-01), None
patent: 0 764 848 (1997-03-01), None
patent: 1 020 532 (2000-07-01), None
patent: 1 555 173 (1979-11-01), None
patent: 8-105875 (1996-04-01), None
patent: 8-105876 (1996-04-01), None
Tanaka A. Postprandial Hyperlipidemia and Remnant Lipoprotein.Arteriosclerosis 25(9, 10)371-6, 1998, translation provided.
Nakajima K. et al. A New Approach for the Detection of Type III Hyperlipoproteinemia by RLP Cholesterol Assay, J of Atherosclerosis and Thrombosis 1(1)30-36, 1994.
Uematsu, et al., “Relation between micellar structure of model bile and activity of esterase”, Biochmica et Biophysica Acta, vol. 1258, No. 2 (1995), pp. 122-134.
Fitzpatrick ,Cella, Harper & Scinto
Gitomer Ralph
Kyowa Medex Co., Ltd.
LandOfFree
Aqueous method for determining cholesterol in remnant-like... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Aqueous method for determining cholesterol in remnant-like..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Aqueous method for determining cholesterol in remnant-like... will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-3721707