Aqueous diafiltration process for preparing acellular vaccines,

Details Aqueous diafiltration process for preparing acellular vaccines, Aqueous diafiltration process for preparing acellular vaccines,

1989-11-13

1993-07-06

Russel, Jeffrey E.

Drug, bio-affecting and body treating compositions

Whole live micro-organism, cell, or virus containing

Genetically modified micro-organism, cell, or virus

424 88, 514 54, 530350, 530395, 530806, 530825, 530419, A61K 3902

Patent

active

052251942

ABSTRACT:
Immunogenic components are recovered from pathogenic bacteria grown on semi-defined, serum-free media in purified forms that are useful as veterinary acellular vaccines and include both polysaccharides as well as proteins. Recovery is accomplished by homogenizing the cells; precipitating unwanted cellular compounds such as nucleic acids using a quaternary ammonium salt to form insoluble ionic complexes; treating the resulting supernatant with a hypersaline solution to dissociate any residual ionic complexes; and concentrating and dialyzing the supernatant to remove the ammonium and chloride salts and various unwanted components derived from the culture medium.

REFERENCES:
patent: 3636192 (1972-01-01), Gotschlich
patent: 4123520 (1978-10-01), Hagopian
patent: 4298597 (1981-11-01), Acres et al.
patent: 4753796 (1988-06-01), Moreno et al.
patent: 4755381 (1988-07-01), Cryz
patent: 4877613 (1989-10-01), Vedros et al.
Adlam D. C., et al. 1984, Journal of Gen. Microbiology 130: 2415-2426.
Gotschlich, E. C. 1975, Monogr. Allergy 9: 245-258.
Hall, Richard F., et al. 1977, Vet. Med./Small Animal Clinician 72: 1368-1370.
McKinney, K. L., et al. 1985, Vet. Microbio. 10: 465-480.
Rosendal, S., et al. 1986, Vet. Microbio. 12: 229-240.
Vedros, N. A., et al., 13th Ann. Conf. and 7th Eastern Fish Health Workshop, Baltimore, Maryland, May 9-13, 1982.
Vedros, N. A., et al. 1988, Diseases of Aquatic Organisms 5: 157-161.
Vedros, N. 1976, WHO Techn. Report Series No. 588.
Knox, K. W., et al. 1960, Immunology 3: 352-362.

Affiliated with

Also associated with

Rating

Aqueous diafiltration process for preparing acellular vaccines, does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Aqueous diafiltration process for preparing acellular vaccines, , we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Aqueous diafiltration process for preparing acellular vaccines, will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1688145