Aqueous cholesterol standard solution and process for its prepar

Details Aqueous cholesterol standard solution and process for its prepar Aqueous cholesterol standard solution and process for its prepar

1987-11-17

1989-09-19

Richman, Barry S.

Chemistry: analytical and immunological testing

Composition for standardization, calibration, simulation,...

Lipid, cholesterol, or triglyceride standard or control

436 18, G01N 3100

Patent

active

048681393

ABSTRACT:
The present invention provides an aqueous cholesterol standard solution with a definite content of cholesterol, wherein it contains a detergent mixture of 10 to 90% of cholic acid and 90 to 10% of desoxycholic acid or of appropriate salts or derivatives of these acids.
The present invention also provides a process for the preparation of this aqueous cholesterol standard solution, wherein a detergent mixture of cholic acid and desoxycholic acid or of appropriate salts and derivatives of these acids is dissolved in distilled water or in 0.9% aqueous sodium chloride solution, an appropriate preservation agent and/or a buffer effective in the pH range of from 7 to 9 optionally added thereto, and a definite, precisely defined amount of cholesterol is dissolved in the solution thus obtained, while stirring and warming to 40.degree. to 60.degree. C.

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"Disolution Rate Behavior of Solid Cholesterol Preparations in Bile Acid Solutions", Chem. Abstract No. 95: 103243x.

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