Aqueous carrier waveguide in a flow cytometer

Details Aqueous carrier waveguide in a flow cytometer Aqueous carrier waveguide in a flow cytometer

1994-04-20

1995-12-12

Gonzalez, Frank

Optics: measuring and testing

For size of particles

By particle light scattering

356338, 356339, 385125, 250574, G01N 1502, G01N 2100, G02B 620

Patent

active

054754879

ABSTRACT:
The liquid of a flow cytometer itself acts as an optical waveguide, thus transmitting the light to an optical filter/detector combination. This alternative apparatus and method for detecting scattered light in a flow cytometer is provided by a device which views and detects the light trapped within the optical waveguide formed by the flow stream. A fiber optic or other light collecting device is positioned within the flow stream. This provides enormous advantages over the standard light collection technique which uses a microscope objective. The signal-to-noise ratio is greatly increased over that for right-angle-scattered light collected by a microscope objective, and the alignment requirements are simplified.

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