Aptamers that bind thrombin with high affinity

Drug – bio-affecting and body treating compositions – Designated organic active ingredient containing – Carbohydrate doai

Reexamination Certificate

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Details

C536S023100, C536S024500

Reexamination Certificate

active

07998939

ABSTRACT:
The invention provides aptamers capable of binding to thrombin useful as therapeutics for and diagnostics of coagulation related disorders and/or other diseases or disorders in which thrombin has been implicated. The invention further provides materials and methods for the administration of aptamers capable of binding to thrombin.

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Fitter, et al., “Deconvolution of a Complex Target Using DNA Aptamers,”J Biol Chem280(40):34193-34201 (2005).
Bock et al., “Selection of single-stranded DNA molecules that bind and inhibit human thrombin,”Nature, 355(6360):564-566, 1992.
Burmeister et al., “2'-deoxy purine, 2'-0-methyl pyrimidine (dRmY) aptamers as candidate therapeutics,”Oligonucleotides, 16(4):337-351, 2006.
Di Giusto et al., “Construction, stability, and activity of multivalent circular anticoagulant aptamers,”Journal of Biological Chemistry, 279(45):46483-46489, 2004.
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Office Communication, issued in European Patent Application No. 06813718.1, dated Sep. 28, 2010.
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Tasset et al., “Oligonucleotide inhibitors of human thrombin that bind distinct epitopes,”Journal of Molecular Biology, 272(5):688-698, 1997.

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