Aptamer selection method

Details Aptamer selection method Aptamer selection method

2004-03-31

2010-06-29

Liu, Sue (Department: 1639)

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

C536S023100, C536S024100, C536S024300, C435S091200

Reexamination Certificate

active

07745607

ABSTRACT:
The present invention provides a method for the selection of aptamer sequences and aptamers identified using the method. The method comprises immobilizing a library oligonucleotide by forming a duplex with an antisense oligonucleotide, reating the duplex molecule with a target and collecting oligonucleotide molecules which dissociate from the duplex structure.

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Hamaguchi et al. “Aptamer Beacons for the Direct Detention of Proteins”. Analytical Biochemistry 294:126-131, 2001.
Davis et al. “Isolation of high affinity GTP aptamers from partially structured RNA libraries”. PNAS 99(18):11616-11621, Sep. 2002.
Jhaveri et al. “In virto selection of signaling aptamers”. Nature Biotechnology 18:1293-1297, Dec. 2000.
Sooter et al. “Toward automated nucleic acid enzyme selection”. Biol. Chem 382:1327-1334, Sep. 2001.

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