Application of novel DNA fragments as a coding sequence for a si

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...

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4352523, 4352542, 4353201, 435325, 536 231, C12N 119, C12N 510, C12N 1563, C12N 1581

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056311443

ABSTRACT:
The invention relates to new DNA fragments and to their application as a DNA coding fragment for a signal peptide which can be used for the secretion of proteins, said peptide including a sequence of amino-acids which show a degree of correspondence of at least 60% with the sequence of amino-acids (I) or (II), preferably with the sequence (II). The sequences (I) and (II) are as follows: (I) Arg-Phe-Ser-Thr-Thr-Leu-Ala-Thr-Ala-Ala-Thr-Ala-Leu-Phe-Phe-Thr-Ala-Ser-Gl n. (II) Arg-Phe-Ser-Thr-Thr-Leu-Ala-Thr-Ala-Ala-Thr-Ala-Leu-Phe-Phe-Thr-Ala-Ser-Gl n-Val-Ser-Ala.

REFERENCES:
patent: 5087613 (1992-02-01), Courtney
Ernst, J.F. DNA 7(5): 355-360 (1988).
Gabrielsen, O.S. "Efficient secretion of human parathyroid hormon by S cerevisiae." Gene 90: 255-262 (1990).
The Journal of Biological Chemistry, vol. 257, No. 24, 25 Dec. 1982 American Society of Biological Chemists, Inc., (Baltimore, MD), J. Kaput et al.,"Nucleotide sequence of the yeast nuclear gene for cytochrome c peroxidase precursor", pp. 15054-1058.
Journal of Bacteriology, vol. 171, No. 11, Nov. 1989, American Society for Microbiology, (Baltimore, MD), F. Klebl et al., "Molecular cloning of a cell wall exo-.beta.-1,3-glucase from Saccharomyces cerevisiae", pp. 6259-6264.

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