Application of luciferase assay for ATP to antimicrobial drug su

Details Application of luciferase assay for ATP to antimicrobial drug su Application of luciferase assay for ATP to antimicrobial drug su

1975-04-30

1977-03-29

Monacell, A. Louis

Textiles: fiber preparation

1951035R, C12K 104

Patent

active

040147452

ABSTRACT:
The susceptibility of bacteria, particularly those derived from body fluids, to antimicrobial agents is determined in terms of an ATP index measured by culturing a bacterium in a growth medium, assaying the amount of ATP in a sample of the cultured bacterium by measuring the amount of luminescent light emitted when the bacterial ATP is reacted with a luciferase-luciferin mixture, subjecting the sample of the cultured bacterium to an antibiotic agent and assaying the amount of bacterial adenosine triphosphate after treatment with the antibiotic by measuring the luminescent light resulting from the reaction, whereby the ATP index is determined from the values obtained from the assay procedures.

REFERENCES:
patent: 3745090 (1973-07-01), Chappelle et al.
patent: 3772154 (1973-11-01), Isenberg et al.
patent: 3940250 (1976-02-01), Plakas et al.

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