Application of antibiotics of the phleomycin family as selection

Details Application of antibiotics of the phleomycin family as selection Application of antibiotics of the phleomycin family as selection

1991-03-01

1992-06-02

Peet, Richard C.

Chemistry: molecular biology and microbiology

Treatment of micro-organisms or enzymes with electrical or...

Modification of viruses

435 34, 435 691, 435 711, 435 91, 4351721, 4352402, 4352523, 435254, 4353201, 435839, 435849, 435878, 435942, 536 27, 935 9, 935 22, 935 55, 935 59, 935 66, 935 69, 935 70, 935 72, C12N 1570, C12N 1564, C12N 1574, C12N 1509

Patent

active

051186209

ABSTRACT:
A method of transforming eukaryotic or prokaryotic hosts sensitive to an antibiotic of the phleomycin family to confer resistance to the antibiotic is disclosed in which a phleomycin resistance gene is used as a selectable marker.

REFERENCES:
Maniatis et al 1982 Molecular Cloning-Cold Spring Harbor Laboratory pp. 11-13.
Suzuki et al. 1969, J. Antibiotics 22(9):446-448.
Putnoky et al. 1983, Mol Gen Genet. 191:288-294.
Gritz et al 1983 Gene 25:179.
Jimenez et al Nature 287:869-871 (1980).
Takeshi Murakami et al, "Cloning of Anti-biotic Resistance Genes in Streptomyces" Journal of Antibiotics, vol. 36, No. 10, Oct. 1983, pp. 1305-1311.
Yasutoshi Takeichi et al, "Cloning of Bacillus subtilis alphaamylase Structural Gene in Plasmid pUB110", Agric. Bid. Chem., vol. 4, No. 1, Jan. 1983, pp. 159-161.
P. Attfield et al., "R Plasmids Mediate Protection and Sensitivity to Bleomycin", J. Pharm. Pharmacol. 1980, 32 (Suppl.), p. 31p.
Takado Iijima et al., "Mutability of the Phleomycin Resistant Mutants of Bacillus subtilis. I. Isolation of Genetically Unstable Mutants," J. Gen. Appl. Microbiol., 1970 vol. 16, No. 5, pp. 419-427.

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