Drug – bio-affecting and body treating compositions – Fermentate of unknown chemical structure – Processes of making – purifying or recovering
Patent
1995-08-21
1998-06-02
Witz, Jean C.
Drug, bio-affecting and body treating compositions
Fermentate of unknown chemical structure
Processes of making, purifying or recovering
4352547, A61K 3572, C12N 114
Patent
active
057595437
DESCRIPTION:
BRIEF SUMMARY
FIELD OF ART
The present invention relates to mycology and medicine and, more particularly, to the strains of fungi-producers of physiologically-active substances and preparations on their base for use in public health services.
BACKGROUND OF THE INVENTION
The strain of a higher fungus INONOTUS OBLIQUUS and the befungin preparation developed on its base are well known to those skill in the art.
Befungin is an extract from the excrescences growing on birch trees under the effect of this fungus. The preparation is a semi-fluid mass containing 30% of dry matter with 1% of cobaltichloride or 1.5% of cobaltisulfate added. It is used as a symptomatic medicine improving the state of health of patients suffering from chronic gastritis and dyskinesia of gastroenteric tract with prevailing atonia (M. D. Mashkovsky, Medicinal Agents, "Meditsina" publishers, Moscow, 1984, Vol. 2, p. 162).
However, the resources of untreated birch fungus for manufacture of befungin are extremely low, same as is the spectrophysiological effect of the preparation.
It is also known that the fungi Lentinus edodes, Ganoderma lucidum, Pleurotis ostreatus produce and antischlerotic effect and influence the content of cholesterin, normalizing its level in the organism (Lee Khva Rei, Vasiliyev L. V., Orekhov L. N., Tertov V. V., Tutyan V. A. "Antischerotic Properties of Higher Fungi". Problems of Alimentation No. 1, 1989, pp 16-20).
However, these fungi are characterized by a low rate of growth (mycelium grows within 5-7 days while its fruit body, over 70 days) and possess a narrow range of physiological effect.
The fungi of Fusarium genus cultivated on the basis of modern biotechnology grow at a higher rate.
Known in the prior art is the strain of fungus Fusarium sambucinum Fuckel var. ossicolum (Berk. et Curt.) Bulai VSB-917--a producer of food or fodder protein (All-Union Collection of Industrial Microorganisms of the VNIIgenetika Institute, collection code VKPM F-169). This fungus, Fusarium sambucinum var ossicolum (Berk. et Curt.) Bulai VSB-917 was deposited in June 1981 with the Russian National Collection of Industrial Microorganism (VKPM) at 1 Poroznny proezd, Moscow, Russia, 113545 with the Accession number given by the International Depository Authority of VKPM F-169.
Formerly, this strain was named Polyporus sp. VSB-917 (Microbiol. Sciences, 1986, v. 3, No. 6, pp 168-171).
The strain Fusarium sambucinum Fuckel var. ossicolum (Berk. et Curt.) Bulai VSB-917 was produced at the active growth stage during continuous cultivation of mycelium of the Fusarium sambucinum PS-64 strain (All-Union Collection of Industrial Microorganisms of the VNIIgenetika Institute, collection code TsMPM-F-165).
The strain Fusarium sambucinum VSB-917, F-169 possesses the following cultural and physiological features: on wort agar it forms white felt colonies which turn pink with age, does not form fruit bodies; commissures not discovered. Characterized by two types of sporification: crescent-shaped conidia with 2-5 partitions, and chamydospores. Being cultivated in a liquid nutrient medium forms a multi-cellular filiform mycelium with ascomycetous partitions and Voronin bodies. In the active growth stage the hyphas are 2.5-3.5 microns thick, the mycelium cells are homogeneous, without lipide and other inclusions, the ends of hyphas (mycelium) are evenly rounded. On aging, the cells develop numerous inclusions of fat.
This strain is an aerobe, grows in a temperature interval of 22.degree.-28.degree. C. and a pH interval of 4.5-7.0, the optimum pH reaching 5.8. Does not dilute gelatine. Assimilates glucose, saccharose, lactose, maltose, galactose, raffinose, as well as starch, ethanol, glycerine, acetic acid, mannit, xylitol.
Attitude towards the source of nitrogen: assimilates ammonium salts, nitrates, urea, peptone.
Under the conditions of submerged cultivation the strain grows well on milk whey (4-6% lactose) with ADM (absolutely dry matter) yield of 20-25 g per one liter of nutrient medium.
The output of the drain-add process is 1.4-1.6 kg/cu m/h.
The myc
Morozov Alexandr Lvovich
Morozova Galina Rostislavovna
Hanley Susan
Krestyanskoe Khozyaistvo "AGROFIRMADIZHA"
Witz Jean C.
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