Multicellular living organisms and unmodified parts thereof and – Method of introducing a polynucleotide molecule into or... – The polynucleotide contains a tissue – organ – or cell...
Reexamination Certificate
2000-04-27
2002-05-21
Benzion, Gary (Department: 1638)
Multicellular living organisms and unmodified parts thereof and
Method of introducing a polynucleotide molecule into or...
The polynucleotide contains a tissue, organ, or cell...
C536S024100, C435S320100, C435S419000, C435S468000, C800S295000
Reexamination Certificate
active
06392122
ABSTRACT:
FIELD OF THE INVENTION
The present invention relates to novel apple fruit-associated and Thi 1.3:actin fusion promoters, and to heterologous nucleic acid constructs, vectors, kits, and transformation methods employing such promoters. The invention further relates to transgenic plant cells and plants transformed with heterologous nucleic acid constructs comprising an apple fruit-associated or Thi 1.3:actin fusion promoter.
REFERENCES
Adams, D. O., and Yang, S. F.,
Plant Physiology
70:117-123 (1977).
An, G, et al.,
EMBO J
. 4:277-284 (1985).
Atkinson et al.,
Plant Mol. Biol
. 38:449-560, 1998.
Ausubel, F M, et al., in CURRENT PROTOCOLS IN MOLECULAR BIOLOGY, John Wiley and Sons,
Inc., Media, Pa. (1992).
Ayub, R., et al.,
Nature Biotechnology
14:862-866 (1996).
Altschul, et al.,
Nucl. Acids Res
. 25(17) 3389-3402 (1997).
Becker, D., et al., Plant Mol. Biol. 20:1195-1197 (1992).
Belanger F C, et al.,
Plant Mol. Biol
. 29: 809-821, 1995.
Bellini, C., et al.,
Bio/Technology
7(5):503-508 (1989).
Bestwick, et al., 1995; U.S. Pat. No.5,859,330
Brunke, K J and Wilson, S L, European Patent Publication No. 0 559 603 A2, published Sep. 08, 1993.
Clendennen and May,
Plant Physiol
115(2):463-9, 1997
Comai, L., and Coning, A. J., U.S. Pat. No. 5,187,267, issued Feb. 16, 1993.
Cordes, S, et al.,
The Plant Cell
1:1025-1034, 1989.
Doerner et al.,
Nature
380: 520-523, 1996.
Dong, J. Z., et al.,
Bio/Technology
9:858-863 (1991).
Fang, G, and Grumet, R,
Plant Cell Rep
. 9:160-164 (1990).
Ferro, A, et al., U.S. Pat. No. 5,416,250, issued May 16, 1995.
Fils-Lycaon et al., Plant Physiol. 111:269-273, 1996.
Fujioka et al.,
Plant Cell
9: 1951-62, 1997.
Good et al.,
Plant Mol. Biol
. 26:781-790, 1994.
Gonsalves, C, et al.,
J Amer. Soc. Hort. Sci
. 119:345-355 (1994).
Hooykaas, P J, and Schilperoot, R A, in TRENDS IN BIOCHEMICAL SCIENCES, International
Union of Biochemistry and Elsevier Science Publishers, v.10(8):307-309 (1985).
Houck, C M and Pear, J R, U.S. Pat. No. 4,943,674, issued Jul. 24, 1990.
Hughes, J A, et al.,
J. Bact
. 169:3625-3632 (1987).
Jacob-Wilk, D. et al.,
Plant Mol. Biol
. 35: 661-666, 1997.
Jefferson, R A, et al.,
EMBO J
: 6:3901 (1987a).
Jefferson, R A,
Plant Mol. Biol. Rep
. 5:387 (1987b).
Jefferson, R A,
Nature
342(6251) 837-838, 1989).
Klein, T. M., et al.,
PNAS USA
85(22):8502-8505 (1988).
Knee M. POME FRUITS In; Seymour et al., Eds., BIOCHEMISTRY OF FRUIT RIPENING, p 325-346, Chapman & Hall, London, 1993.
Lay-Yee, 1993
, Plant Physiol
. 103: 1017)
Lee S A, et al.
Plant Physiol
. 103(3): 1017, 1993.
Ledger and Gardner,
Plant Mol. Biol
. 25:877-886, 1994.
Leisner, S. M., and Gelvin, S. B.,
Proc. Natl. Acad. Sci. USA
85(8):2553-2557 (1988).
Li et al.,
Science
272: 398-401, 1996.
Lin, E., et al.,
Plant Mol. Biol
. 23:489-499 (1993).
Maniatis, T et al., in MOLECULAR CLONING: A LABORATORY MANUAL, Cold Spring Harbor
Laboratory, Cold Spring Harbor, N.Y. (1982).
Mathews H et al.,
Plant Cell Rep
., 14:471-476, 1995a.
Mathews H et al.,
In vitro
31:36-43, 1995a.
McCormick et a,
Plant Cell Reports
5:81-84, 1986.
Miki, B L A, et al., PLANT DNA INFECTIOUS AGENTS (Hohn, T., et al., Eds.) Springer-Verlag,
Vienna, Austria, pp. 249-265 (1987).
Ni, M et al.,
Plant J
. 7:661-676 (1995).
Norelli et al.,
HortScience
, 31:1026-1027, 1996.
Picton S, et al.,
Plant Physiology
103(4):1471-1472 (1993).
Ranier et al.,
Bio/Technology
8:33-38, 1990.
Ribeiro A et al.,
Plant J
10:361-8, 1996.
Robinson H L and Torres, Calif.,
Sem. Immunol
. 9:271-282, 1997.
Rogers S, U.S. Pat. No. 5,034,322, issued Jul. 23, 1991.
Sagi et al.,
Bio/Technology
5:481-485, 1995.
Sambrook J, et al., in MOLECULAR CLONING: A LABORATORY MANUAL, Cold Spring
Harbor Laboratory Press, Cold Spring Harbor, N.Y., Vol. 2 (1989).
Sung and An, 5th International Congress of Plant Molecular Biology, Singapore, Poster Abstract #403, 1997; GenBank accession number U78948.
Tattersall D B, et al.,
Plant Physiol
114: 759-69 (1997).
Valles M P and Lasa, J M,
Plant Cell Rep
. 13:145-148 (1994).
Van Haaren M J J, et al.,
Plant Mol. Bio
. 21:625-640 (1993).
Verdaguer et al.,
Plant Mol Biol
. 37:1055-1067 (1998).
Xu R, et al.,
Plant Mol. Biol
. 31:1117-1127(1996).
Yao J et al.,
J. Amer. Hort. Sci
. 124(1):8-13, 1999.
Yoshioka K, et al.,
Jpn. J. Breeding
42(2):278-285 (1992).
Zhu Q, et al.,
Plant Cell
7:1681-1689 (1995).
BACKGROUND OF THE INVENTION
Transcriptional regulatory sequences or promoters that regulate gene expression in plants are essential elements of plant genetic engineering. Several examples of promoters useful for the expression of heterologous genes in plants are now available (Zhu, et al., 1995; Ni, et al., 1995).
Most promoters are from about 500-1500 bases. Promoters for expressing a heterologous gene sequence in plants can be derived from plant DNA, e.g., the cauliflower heat shock protein 80 (hsp80, Brunke and Wilson, 1993; U.S. Pat. No. 5,612,472), or from other sources, for example, plant viruses e.g., the 35S cauliflower mosaic virus promoter, or bacteria which infect plants, e.g., the nopaline synthase (nos) promoter (Rogers, 1991), the octopine synthase (ocs) promoter (Leisner and Gelvin, 1988) and the mannopine synthase (mas) promoter from Agrobacterium.
Expression of heterologous genes or selected sequences of genes in transgenic plants has typically involved the use of constitutive promoters, which drive the expression of a product throughout the plant at all times and in most tissues (e.g., hsp80), the tomato ubiquitin promoter (Picton, et al., 1993), and the raspberry E4 promoter (U.S. Pat. Nos. 5,783,393; and 5,783,394).
A limited number of inducible and/or tissue specific promoters are known. Promoters that provide fruit-specific expression include the E4 and E8 promoter from tomato (Cordes, et al., 1989; Bestwick, et al., 1995; U.S. Pat. No. 5, 859,330). Another fruit-specific promoter is the tomato 2AII gene promoter. It has been demonstrated that nucleic acid sequences placed under the regulatory control of the 5′ non-coding region of the tomato 2AII gene (Van Haaren, 1993) are preferentially transcribed in developing fruit tissue. Fruit specific regulation of the kiwifruit actinidin promoter has been reported to be conserved in transgenic petunia plants (Lin, et al., 1993).
Differential screening has been used to identify abundant transcripts in developing and ripening fruit. In banana, for example, a cDNA clone encoding a putative thaumatin-like protein is identified as among the most abundant transcripts in ripening fruit, and in kiwifruit a metallothionein-like transcript is identified as very abundant in ripening fruit (Clendennen and May, 1997; Ledger and Gardner, 1994). Abundant transcripts have also been identified in the fruit of grape, cherry, and apple (Fils-Lycaon et al., 1996; Lee et al., 1993).
A transcript was previously identified in Golden Delicious apple (GenBank L15194; Lee, Gardner, and Lay-Yee, 1993
, Plant Physiol
. 103: 1017) that is abundant in fruit and shows sequence similarity to an auxin-repressed protein (ARP) of unknown function from strawberry.
Apple is a fruit which has been the subject of a great deal of study over the past several decades (Knee, 1993). Ethylene reduction is desired by packers and shippers in order to maintain apples from over-ripening and rotting. Refrigeration, high concentrations of CO
2
and low concentrations of O
2
are currently being employed to reduce the harmful effects of ethylene during storage. Such methods suffer from the disadvantages that fruits picked at preclimacteric stages (prior to full ripening) respond better to controlled atmosphere conditions than those at mature stages and many varieties of apple suffer from chilling injury and physiological disorders due to controlled atmosphere conditions, rendering them unmarketable.
Ethylene is a plant hormone influencing many aspects of plant growth and development, and is known to play a major role in the ripening process in fruits and vegetables. A large amount of ethylene is also produced following trauma caused by chemicals, temperatur
Clendennen Stephanie K.
Schuster Debra K.
Agritope, Inc.
Benzion Gary
Judge Linda R.
Kruse David H
LandOfFree
Apple promoters for expression of transgenes in plants does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Apple promoters for expression of transgenes in plants, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Apple promoters for expression of transgenes in plants will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-2883583