Apparatus kit for detecting Dientamoeba fragilis

Chemical apparatus and process disinfecting – deodorizing – preser – Analyzer – structured indicator – or manipulative laboratory... – Sample mechanical transport means in or for automated...

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435 29, 435 30, 435 34, 435810, 435 4051, 4352887, 4352871, G01N 3700, G01N 3348, C12Q 104

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active

054806135

ABSTRACT:
A method and apparatus for producing detectable intestinal parasites. The method includes obtaining an intestinal mucosa sample (e.g. feces) having intestinal parasites, such as Dientamoeba fragilis; and contacting the obtained intestinal mucosa sample with an acridine base compound (e.g. acridine orange and/or acridine yellow, etc.) such that the intestinal parasites become differentially stained and detectable by a human eye when viewed through a fluorescence microscope. The apparatus includes a kit or the like which includes at least one vessel or vial. Preferably, two vials are contained within the kit with one vial having an isotonic salt solution including a salt, sodium chloride, potassium phosphate, etc., and the other vial containing an acridine biological staining compound.

REFERENCES:
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patent: 4376820 (1983-03-01), Giannini et al.
patent: 4674640 (1987-06-01), Asa et al.
Diagnostic Parasitology, Clinical Laboratory Manual, 2nd edition, 1979, pp. 99-101 and 105.
Medical Parasitology, A Self-Instructional Text, 1979, pp. 158-159.
Clinical Diagnosis & Management by Laboratory Methods-16th edition, 1979, pp. 1759-1765 and 1769.

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