Chemistry: molecular biology and microbiology – Apparatus – Including measuring or testing
Patent
1991-07-22
1995-02-07
Naff, David M.
Chemistry: molecular biology and microbiology
Apparatus
Including measuring or testing
435174, 435178, 435182, 4352401, 43524022, 4352521, 4352541, 435288, C12M 100, C12M 140, C12N 1110, C12N 500
Patent
active
053875222
ABSTRACT:
Biological material is entrapped in a hydrophilic gel by using an apparatus containing a biphasic spray head. In a preferred embodiment, mammalian cells are mixed with a solution of alginate to form a mixture and the mixture is fed to a biphasic spray head where the mixture passes through a nozzle surrounded by an annular passageway through which air is passed. Droplets of the mixture are formed at the tip of the nozzle and air passing through the passageway frees the droplets from the tip and propels them into the atmosphere in the form of fine spherical droplets. The droplets then contact a solution of divalent cation such as calcium chloride which gels the alginate. Preferably, the nozzle has an inner diameter of between about 0.006" and 0.016" and is beveled at an angle between about 15.degree. and 30.degree. to form a conical tip.
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Frye Maureen E.
Lynch Maurice M.
Vasington Paul J.
Boxer Matthew
Dicker Eric S.
Maitner John J.
Naff David M.
Schering Corporation
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