Apparatus for visible, preparative column chromatography

Liquid purification or separation – With means to add treating material – Chromatography

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210656, B01D 1508

Patent

active

061561967

ABSTRACT:
The presented invention relates to an apparatus and process, which provides a suitable application for preparative separation of organic mixtures by flash and/or vacuum liquid chromatographic techniques. In particular when a quartz tubing is used as the body of the column and fluorescent adsorbent as packing material, the apparatus and process allows the operator to very simply and inexpensively assess the separation of a mixture of UV-active organic compounds being eluted through it by projection of a UV-light is projected on it allowing the position of the UV-active material on the fluorescent adsorbent in the column to become visible. Therefore, the degree of separation of the UV-active materials can be directly monitored through the column by user without the use of detectors, presenting real-time optimization of solute resolution during the separation process, prior to collection. The apparatus consists of a solvent reservoir, chromatographic column, a open shelter for glass or quartz tubing, and adapters. A solvent reservoir is comprised of a container with a cap having two (or three) connections: one for transfer of eluant and one for introduction of air for vacuum chromatography or compressed air for flash chromatography and one for fine adjustment of compressed air flow.

REFERENCES:
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patent: 4591442 (1986-05-01), Andrews
patent: 4774686 (1988-09-01), McClary
patent: 4812393 (1989-03-01), Goswami
patent: 5378361 (1995-01-01), Baeckstrum
patent: 5888829 (1999-03-01), Gee
Fischer, Anal. Biochem. (1964), 9: 303-307.
Loev, Chemistry and Industry, London, Dec. 1967 pp. 2062-2032.
Still, J. Org. Chem. (1978), 43: 2923-2925.
Targett., J. Org. Chem. (1979), 44: 4962-4964.

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