Chemistry: molecular biology and microbiology – Apparatus – Bioreactor
Patent
1978-03-14
1981-01-27
Wiseman, Thomas G.
Chemistry: molecular biology and microbiology
Apparatus
Bioreactor
435 14, 435 18, 435 25, 435 27, C12M 134
Patent
active
042476470
ABSTRACT:
A method for the quantitative determination of sugars in a fluid sample. The sugar sample, which may contain free glucose, is passed through a scavenger stage where substantially all of the glucose is removed, so that it does not interfere with the determination. The glucose-free sample is then passed through an immobilized glucose generating stage to produce glucose in molar proportion to the total initial concentration of the sugars present. The generated glucose is passed through an immobilized hydrogen peroxide generating stage to form gluconic acid and hydrogen peroxide. The generated hydrogen peroxide is detected in suitable detection means, typically a polarographic cell. Total free glucose is determined by measuring the total sugars in the sample by bypassing the scavenger stage, determining total sugar, then determining the glucose in the sample without bypassing the scavenger and measuring free glucose as the difference.
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Levin et al., "High-Precision Industrial Measurements with Immobilized Enzymes", presented at American Institute of Chem. Engineers, 77th National Meeting, Pittsburgh, Pa., 1974.
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Barabino Raymond C.
Keyes Melvin H.
Technicon Instruments Corporation
Tedesco S. P.
Wiseman Thomas G.
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