Chemistry: molecular biology and microbiology – Apparatus – Bioreactor
Patent
1978-05-12
1979-10-02
Shapiro, Lionel M.
Chemistry: molecular biology and microbiology
Apparatus
Bioreactor
435 22, 435808, 204195B, 204 1T, G01N 3114
Patent
active
041697650
ABSTRACT:
This invention relates to a method and apparatus for rapid quantitative determination of .alpha.-amylase in aqueous samples such as blood serum, etc. The method comprises a flow-through of the sample through various immobilized reagents contained in sequential stages. A scavenger stage initially removes glucose originally present in the sample and comprises immobilized glucose oxidase and catalase. The glucose-free sample flows through an immobilized starch stage substrate preferably containing a high percentage of amylose to quantitatively react with the .alpha.-amylase in the sample and produce oligosaccharides. The sample with the oligosaccharides flows through a glucose-generating stage containing immobilized glucoamylase which converts the oligosaccharides to glucose. The glucose-containing sample enters a detection stage wherein the glucose is converted to H.sub.2 O.sub.2 by flowing through immobilized glucose oxidase, and the H.sub.2 O.sub.2 produced is quantitatively detected in detection means such as a polarographic cell. The immobilized reagents are preferably contained in an apparatus which comprises at least one column in combination with the detection means and a readout means.
REFERENCES:
patent: 4000042 (1976-12-01), Adams
Wilson et al., Clinical Chemistry, vol. 21, No. 7, pp. 947-948, (item 36), 1975.
Ceska et al., Clinical Chim. Acta., vol. 26, pp. 437-444 (1969).
Shapiro Lionel M.
Technicon Instruments Corp.
Tedesco S. P.
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