Apparatus for testing MAI (mycobacterium avium-intracellulare) f

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving viable micro-organism

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435 29, 435863, 435864, C12Q 118

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active

055695922

ABSTRACT:
An apparatus for determining the sensitivity of MAI to different antimicrobial agents and dosages thereof is provided. The apparatus comprises a plurality of test tubes adapted to contain an amount of an antimicrobial agent to be tested and MAI complex organisms to be assayed and a separate paraffin coated slide adapted for placement in each of the test tubes. The growth of the MAI complex organisms on the slide can be used to determine the concentration of the antimicrobial agent necessary to resist MAI complex organism growth on the slide. An associated method is also disclosed.

REFERENCES:
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Mishra, S. K. et al., "Observations On Paraffin Baiting As A Laboratory Diagnostic Procedure in Nocardiosis", Mycopathologica and Mycologia Applicatia 51 (2-3):147-157 (1973).
Ollar, R.-A., "A Paraffin Baiting Technique that Enables a Direct Microscopic View of in situ Morphology of Nocardia asteroides with the Acid-Fast or Fluorescence Staining Procedures", Zbl. Bakt. Hyg., Abt. Orig. A, 234:81-90 (1976).
Kirihara, J. M. et al., "Improved Detection Times for Mycobacterium avium Complex and Mycobacterium tuberculosis with BACTEC Radiometric System", J. Clin. Microbiol. 22:841-845 (1985).
Gonzalez, R. et al., "Evaluation of Gen-Probe DNA Hybridization Systems for the Identification of Mycobacterium tuberculosis and Mycobacterium avium-intracellulare", Diagn. Microbiol. Infect. Dis. 8:69-77 (1987).
Wallace, J. M. et al., "Mycobacterium avium Complex Infection in Patients with Acquired Immunodeficiency Syndrome--A Clinicopathologic Study", Chest 93(5): 926-932 (1988).

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