Apparatus for separation of nucleated blood cells from a blood s

Chemistry: electrical and wave energy – Apparatus – Electrophoretic or electro-osmotic apparatus

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204450, 204518, 204543, 204600, 204630, 2101982, 210209, 210456, G01N 2726, G01N 27447

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059067241

ABSTRACT:
A charge-flow separation apparatus (CFS) for enriching rare cell populations, particularly fetal cells, from a whole blood sample by separating the rare cell fractions from whole fractions according to the relative-charge density and/or the relative binding affinity for a leukocyte depletion solid phase matrix is described. The apparatus having an internal cooling system allows for dissipating heat generated by the electric field of the apparatus. The internal cooling system, consisting of a plurality of cooling tubes to circulate coolant material, prevents cellular degradation typically associated with the high heat generated by the electric field and permits the use of a higher voltage gradient to shorten separation times.

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