Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1994-08-30
1997-12-16
Hutzell, Paula K.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
422 56, 422 57, 422 58, 422 61, 422 681, 435 71, 4352871, 4352872, 4352877, 4352878, 435805, 435810, 436514, 436518, 436524, 436527, 436528, 436530, 436805, C12Q 168, G01N 33543, G01N 33548, G01N 33552
Patent
active
056983956
ABSTRACT:
This disclosure describes an apparatus for separation and detection of target molecules in a liquid sample containing the target molecules and non-target molecules comprising at least one absorption area, a bibulous carrier defining a first liquid transport path in fluid contact with at least one absorption area including at least one reaction area upstream of the at least one absorption area wherein the reaction area includes a capture reagent and a sample area upstream of the reaction area wherein the liquid sample when applied to sample area is transported by capillary action past the reaction area where at least a portion of the target molecules may be bound to the capture reagent to permit detection of the target molecules. The liquid sample is then transported by capillary action on to the absorption area. A second liquid transport path is in fluid communication with the reaction area of the bibulous carrier for transporting a washing solution applied to the reaction area to the at least one absorption area thereby removing interfering substances from the reaction area. Means are also provided for alternatively selecting between the first and second liquid transport path during capillary transport of the liquid sample.
REFERENCES:
patent: 4522923 (1985-06-01), Deutsch et al.
patent: 4857453 (1989-08-01), Ullman
patent: 5114673 (1992-05-01), Berger et al.
patent: 5232663 (1993-08-01), Wilk et al.
patent: 5244631 (1993-09-01), Morikawa
Alajem Sara
Reinhartz Avraham
Ritterband Menachem
Rusu Ileana
Schrift Michal
Grun James L.
Hutzell Paula K.
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