Apparatus for sensitive detection and quantitative analysis of b

Details Apparatus for sensitive detection and quantitative analysis of b Apparatus for sensitive detection and quantitative analysis of b

1977-06-16

1978-09-19

Willis, Davis L.

Radiant energy

Luminophor irradiation

With ultraviolet source

G01N 2138

Patent

active

041156994

ABSTRACT:
Apparatus is disclosed for sensitively detecting and quantitatively analyzing biological and biochemical substances. Charged into a cell is a test molecule labeled with a fluorescent dyestuff. An exciting optical system transmits plane polarized light from a light source into the labeled molecule to excite the molecule, and a polarizing optical system takes up fluorescent light emitted from the molecule. A photomultiplier is arranged to measure the fluorescence intensities of parallel and perpendicularly polarized light from the molecule. A calculator coordinated with the polarizing optical system computes and converts the fluorescence intensities into the degree of polarization of the molecule.

REFERENCES:
patent: 3886363 (1975-05-01), Ohnishi et al.
patent: 3967902 (1976-07-01), Steinberg

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