Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1997-12-12
1998-11-10
Gitomer, Ralph
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
4352887, 935 77, C12Q 168, C12M 140
Patent
active
058342044
ABSTRACT:
The detectability of luminescent molecules in solution is enhanced by reducing the background luminescence due to impurity species also present in the solution. A light source that illuminates the solution acts to photolyze the impurities so that the impurities do not luminesce in the fluorescence band of the molecule of interest. Molecules of interest may be carried through the photolysis region in the solution or may be introduced into the solution after the photolysis region.
REFERENCES:
patent: 4793705 (1988-12-01), Shera
patent: 4962037 (1990-10-01), Jett et al.
patent: 5215883 (1993-06-01), Chu
patent: 5424186 (1995-06-01), Fodor et al.
Ambrose W. P., Single Molecule Detection and Photchemistry on a Surface Using Near Optical Excitation, Physical Review Letters 72(1):160-163, Jan. 1994.
Goodwin P., DNA Sequencing by Single Molecule Detection of Labeled Nucleotides Sequentially Cleaved from a Single Strand of DNA, SPIE vol. 1891, 127-131, 1993.
Jong Hoon Hahn et al., "Laser-Induced Fluorescence Detection of Rhrodamine-6G at 6.times.10.sup.15 M," 45 Applied Spectroscopy, No. 5, pp. 743-746 (1991).
J. C. White et al., "Photostability Studies of Phycobiliprotein Fluorescent Labels," 161 Analytical Biochemistry, pp. 442-452 (1987).
Steven A. Soper et al., "The Photophysical Constants of Several Fluorescent Dyes Pertaining to Ultrasensitive Fluorescence Spectroscopy," 57 Photochemistry and Photobiology, No. 6, pp. 972-977 (1993).
Peter M. Goodwin et al., "Ultrasensitive Detection of Single Molecules in Flowing Sample Streams By Laser-Induced Fluorescence," 1985 SPIE Ultrasensitive Laboratory Diagnostics, pp. 79-89 (1993).
E. Brooks Shera et al., "Detection of Single Fluorescent Molecules," 174 Chemical Physics Letters No. 6, pp. 553-557 (1990).
Affleck Rhett L.
Ambrose W. Patrick
Demas James N.
Goodwin Peter M.
Johnson Mitchell E.
Gitomer Ralph
The Regents of the University of California
Wilson Ray G.
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