Apparatus for rapid toxicity testing of a liquid sample

Details Apparatus for rapid toxicity testing of a liquid sample Apparatus for rapid toxicity testing of a liquid sample

1994-09-15

1995-10-17

Beisner, William H.

Chemistry: molecular biology and microbiology

Apparatus

Including measuring or testing

435808, 4352887, 422 64, 2504612, C12M 134, C12Q 102, G01N 2164

Patent

active

054590701

ABSTRACT:
A toxicity testing assay wherein a test sample is prepared with electrochromic dye and a living organism of the cladoceran order or other test organisms, and the sample is irradiated with alternately blue and yellow light to excite fluorescence. The successive groups of fluorescent emissions are then viewed at 90.degree. by a photomultiplier which develops equivalent alternate count outputs, and the count output is amplified and processed to develop data indicating (1) membrane potential of cells of the living organisms and (2) the total dye fluorescence which provides indication of deleterious effects to the organisms.

REFERENCES:
patent: 4548907 (1985-10-01), Seitz
patent: 4811218 (1989-03-01), Hunkapiller
patent: 4977325 (1990-12-01), Bowen
patent: 5042494 (1991-08-01), Alfano
patent: 5094944 (1992-03-01), Hayes
Sanchez P., Toxicity Assessment of Industrial . . . Tox Assess. An Int J., vol. 3 55-80 (1988).
Montana V., Dual-Wavelength Ratiometric . . . Biochem 1989 28 4536-4539.

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